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首页> 外文期刊>Genes and genomics >Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp durum) from a semi-arid region
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Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp durum) from a semi-arid region

机译：半干旱地区硬质小麦（Triticum turgidum ssp durum）与耐盐性相关的SCAR标记的开发

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Durum wheat (Triticum turgidum ssp. durum) is one of the main species of cultivated wheat. In arid and semi-arid areas, salinity stress reduces durum wheat productivity. This study used 26 durum wheat accessions from semi-arid regions in Tunisia to analyze plant tolerance to salt stress. Salt stress was experimentally applied by regularly submerging pots in NaCl solution. The salt tolerance trait index (STTI) and salt tolerance index (STI) of various growth parameters were used as criteria to select for salt tolerance. Analysis of genetic relationships was carried out to determine the genetic distance between durum wheat accessions. Based on simple sequence repeats analysis, a molecular marker for salt stress resistance in durum wheat was developed. Salt-treated plants had reduced morphological traits compared to control plants. Most STTIs in all genotypes were below 100 %. Based on STI, 8 accessions were found to be salt-resistant, 16 were salt-moderate, two were salt-susceptible. Analysis of the genetic relationships among 28 Tunisian durum wheat accessions revealed that landraces of the same nominal type are closely related. Of the 94 SSR primers investigated, three were selected and used to design sequence characterized amplified region (SCAR) primers. One SCAR primer pair, KUCMB_Xgwm403_2, produced a 207 bp band that was present in salt-resistant durum wheat lines but absent in salt-susceptible lines. The results suggest that KUCMB_Xgwm403_2 could be a potential genetic tag for salt-tolerant durum wheats.
机译：硬粒小麦（Triticum turgidum ssp。durum）是栽培小麦的主要品种之一。在干旱和半干旱地区，盐分胁迫降低了硬粒小麦的生产力。这项研究使用了来自突尼斯半干旱地区的26种硬质小麦品种来分析植物对盐胁迫的耐受性。通过定期将盆浸没在NaCl溶液中，通过实验施加盐胁迫。各种生长参数的耐盐性状指数（STTI）和耐盐性指数（STI）被用作选择耐盐性的标准。进行了遗传关系分析以确定硬粒小麦种质之间的遗传距离。基于简单的序列重复分析，开发了硬粒小麦抗盐胁迫的分子标记。与对照植物相比，盐处理的植物具有降低的形态性状。所有基因型的大多数STTI均低于100％。基于性传播感染，发现8个抗盐材料，中度盐16个，盐敏感性2个。对28个突尼斯硬质小麦品种的遗传关系进行分析后发现，相同标称类型的地方品种密切相关。在研究的94个SSR引物中，选择了三个，用于设计序列特征性扩增区（SCAR）引物。一对SCAR引物KUCMB_Xgwm403_2产生了一条207 bp的条带，该条带存在于耐盐硬质小麦品种中，但在盐敏感品种中不存在。结果表明，KUCMB_Xgwm403_2可能是耐盐硬质小麦的潜在遗传标签。

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