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首页> 外文期刊>Genes and genomics >Genome-wide identification of the class III aminotransferase gene family in rice and expression analysis under abiotic stressATIII gene family in japonica and indica rice
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Genome-wide identification of the class III aminotransferase gene family in rice and expression analysis under abiotic stressATIII gene family in japonica and indica rice

机译：水稻Ⅲ类转氨酶基因家族的全基因组鉴定及非生物胁迫下粳稻和in稻ATIII基因家族的表达分析

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摘要

Aminotransferases are pyridoxal 5'-phosphate-dependent enzymes that play crucial roles in plant growth, development, and responses to abiotic stress. The class III aminotransferase family (ATIII family) is an important subfamily. However, no characterization of rice ATIII genes has been previously reported. Using available rice genome sequence information, we identified 12 japonica and 13 indica ATIII genes that were randomly localized on chromosomes 2, 3, 4, 5, 7, 8, and 11. Information provided bythe Plant Genome Duplication Database revealed that four japonica and four indica ATIII genes are the results of segmental duplications, and two japonica and six indica genes resulted from tandem duplications. A phylogenetic analysis of the A77// genes in japonica, indica and Ara-bidopsis enabled the classification of the genes into six different groups, and the characteristics were established before the monocot-dicot and japonica-indica split. An analysis of the Ka/Ks, divergence time and average indel length suggested the diverse selection styles of the duplicated gene pairs. Gene structure and motif analyses revealed that the ATIII gene family has experienced extensive divergence. Real-time PCR was performed to examine the expression pattern of thejaponica ATIII genes in response to various abiotic stresses including drought,salt, and cold. The results suggested that most of the genes were differentially up- or down-regulated in rice seedlings in response to at least one stress factor, which indicates the key role of the rice ATIII gene family in responding to abiotic stresses. These results provide a basis for elucidating the roles of the ATIII genes and their further functional analysis under abiotic stresses.
机译：氨基转移酶是吡咯醛5'-磷酸依赖性酶，在植物生长，发育以及对非生物胁迫的响应中起关键作用。 III类氨基转移酶家族（ATIII家族）是重要的亚家族。然而，以前没有报道水稻ATIII基因的表征。利用可获得的水稻基因组序列信息，我们鉴定了12个粳稻和13个in稻ATIII基因，它们随机位于2、3、4、5、7、8和11号染色体上。植物基因组复制数据库提供的信息显示，四个粳稻和四个III稻ATIII基因是节段重复的结果，两个粳稻和六个in稻基因是串联重复的结果。通过对粳稻，in稻和Ara拟南芥中A77 //基因的系统发育分析，可以将这些基因分为六个不同的组，并在单子叶植物和粳稻-印度ica分裂之前建立了特征。对Ka / Ks，发散时间和平均插入缺失长度的分析表明，重复的基因对具有多种选择方式。基因结构和基序分析表明，ATIII基因家族经历了广泛的分歧。进行了实时PCR，以检测日本粳稻ATIII基因对各种非生物胁迫（包括干旱，盐和寒冷）的响应。结果表明，大多数基因响应至少一种胁迫因子而在水稻幼苗中差异上调或下调，这表明水稻ATIII基因家族在响应非生物胁迫中的关键作用。这些结果为阐明ATIII基因的作用及其在非生物胁迫下的进一步功能分析提供了基础。

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