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NEUTRAL COMET PROFILES: RELIABLE SYSTEM FOR ANALYSES OF DNA STRAND BREAKS DISTRIBUTION

机译:中性基因特征:DNA链断裂分布分析的可靠系统

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摘要

This paper reports the conversion of 2-dimensional to 3-dimensional comet profiles produced by different mutagens able to induce DNA double-strand breaks - radiomimetic agent (bleomycin), restriction endonuclease (Eco RI), ionizing (gamma rays and 7Li-ion beam) and non-ionizing (UVC) radiation. The 2-dimensional comet profiles obtained after application of the neutral comet assay were analyzed using ImageJ software. The obtained data reveal that the 3-dimensional comet profiles can be utilized as a reliable hallmark of the predominant type of DNA breaks induced by agents with different mode of action.
机译:本文报道了由能够诱变DNA双链断裂的诱变剂产生的2维到3维彗星轮廓的转换-放射模拟剂(博来霉素),限制性内切核酸酶(Eco RI),电离(γ射线和7Li离子束) )和非电离(UVC)辐射。应用ImageJ软件分析应用中性彗星试验后获得的二维彗星轮廓。获得的数据表明,三维彗星剖面可以用作具有不同作用方式的试剂诱导的主要DNA断裂类型的可靠标志。

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