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首页> 外文期刊>Genetics and Plant Physiology >SUCROSE-RELATED EFFECTS ON SHOOT DEVELOPMENT AND DOUBLE-PHASE CULTIVATION OF RUSCUS ACULEATUS L.
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SUCROSE-RELATED EFFECTS ON SHOOT DEVELOPMENT AND DOUBLE-PHASE CULTIVATION OF RUSCUS ACULEATUS L.

机译：蔗糖对猕猴桃幼芽发育和双相栽培的影响

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摘要

Ruscus aculeatus is an economically important plant species with remarkable shade and drought tolerance. Its slow growth habit and specific growing requirements limit its frequent field cultivation. Furthermore, micropropagation of R. aculeatus is greatly influenced by determined shoot growth and choice of the source material (origin and explant type). This paper presents our experiments to stimulate the propagation and growth of R. aculeatus shoots by modification of the carbon source (sucrose) andcultivation in a double-phase system. The propagation rate and shoot development were assessed and genome-size stability of the regenerants was verified by flow cytometry. The excess of sucrose in the media prevented shoot proliferation and development.The lowest concentrations were more suitable for shoot induction and even for root growth, contrasting with the general view that the carbon source stimulates the growth in rhizomatous species. The obtained shoots performed a normal morpho-physiologicalhabitus mostly with non-branched stems with typical cladodes. The unaffected genome size status of the regenerants was also confirmed. Double-phase cultivation was effective only in a medium without growth regulators which supported better shoot growth.Addition of paclobutrazol was beneficial both in agar and double-phase cultures producing 5.8 and 3.5 shoots per explant, respectively. However, the propagation rates were not higher than those on agar. The obtained results give useful insights in the micropropagation of R. aculeatus that could reduce production and conservation costs.
机译：刺槐属（Ruscus aculeatus）是具有重要遮荫和耐旱性的重要经济植物。它生长缓慢，习惯和特殊的生长要求限制了其频繁的田间耕作。此外，刺梨的微繁殖受到确定的枝条生长和来源材料（起源和外植体类型）选择的极大影响。本文介绍了我们的实验，通过改变碳源（蔗糖）和在双相系统中进行培养来刺激刺梨幼芽的繁殖和生长。评估繁殖速率和枝条发育，并通过流式细胞仪验证再生子的基因组大小稳定性。培养基中过量的蔗糖阻止了枝条的繁殖和发育。最低的浓度更适合枝条的诱导甚至根的生长，这与一般认为碳源刺激根瘤菌种生长的观点相反。所获得的枝条表现出正常的形态生理习性，主要具有典型分支的非分支茎。还确认了再生体的基因组大小不受影响。双相培养仅在无生长调节剂的培养基中有效，该生长调节剂支持更好的芽生长。添加多效唑在琼脂和双相培养中均有益，每个外植体分别产生5.8和3.5芽。但是，繁殖率不高于琼脂。所获得的结果为刺梨的微繁殖提供了有用的见解，可以减少生产和保存成本。

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