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首页> 外文期刊>Gesunde Pflanzen >Comparison of techniques for detection of potato cyst nematodes (PCN) with regard to quality assurance of regulated pests.
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Comparison of techniques for detection of potato cyst nematodes (PCN) with regard to quality assurance of regulated pests.

机译：就限定有害生物的质量保证而言，检测马铃薯囊肿线虫（PCN）技术的比较。

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摘要

Testing for potato cyst nematode (PCN) is indispensable in EC seed potato production, to avoid a further spread or introduction of this pest. Until now, methods for sampling and detection are varying between member states and even within Germany. Therefore, in order to obtain comparable results within a new PCN directive the procedure will be regulated by EPPO standard diagnostic protocols, like it is the case for testing Globodera spp. resistance of potato varieties. The Bavarian plant protection service hopes, that beside cyst extraction by flotation the bioassay will be part of the new EPPO standard. The bioassay, in contrast to PCR methods, is well established and allows a reliable discrimination not only of PCN species but also of races and pathotypes. Results of a long-time survey demonstrate clearly that bioassay and cyst extraction yield comparable results provided quality assurance criteria are met..
机译：在EC种马铃薯生产中，必须进行马铃薯囊肿线虫（PCN）的测试，以避免进一步传播或引入这种有害生物。到目前为止，取样方法和检测方法在成员国之间甚至在德国内部都不同。因此，为了在新的PCN指令中获得可比的结果，该过程将受EPPO标准诊断协议的约束，就像测试Globodera spp的情况一样。马铃薯品种的抗性。巴伐利亚州植物保护局希望，除了通过浮选提取囊肿以外，生物测定还将成为新的EPPO标准的一部分。与PCR方法相比，该生物测定法已得到充分确立，不仅可以可靠地区分PCN物种，而且还可以区分种族和病原体。长期调查的结果清楚地表明，只要符合质量保证标准，生物测定法和囊肿提取法就可以获得可比的结果。

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