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Research on Verticillium Wilt in Hop Growing Area Hallertau

机译:啤酒花种植区哈勒陶黄萎病研究

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Verticillium wilt is a devastating disease in hops and causes considerable crop losses. V. albo-atrum is the most widespread species, whereas V. dahliae is rarely found. Due to the survival of the fungus for several years in soil by resting structures and the lack of effective chemical control, a fast detection method is needed. One aim of this study was to evaluate the occurrence of Verticillium spp. in the Hallertau region (Germany). Therefore, 76 hop plants, with or without wilt symptoms, were analyzed using a real time PCR (polymerase chain reaction) assay. V. albo-atrum was identified in 57 bine samples. The pathogen can sporadically appear without showing visual symptoms. The abundance of the fungus inside the plants was analyzed. Furthermore, four beneficial bacteria, Burkholderia terricola ZR2-12, Pseudomonas poae RE*1-1-14, Serratia plymuthica 3Re4-18, and Stenotrophomonas rhizophila DSM14405(T), were evaluated as biological control agents in hops. The colonization competence in the root system, plant growing promotion, and antagonistic effects were assessed. All strains colonized the endorhiza and the rhizosphere. The bacterial strains B. terricola ZR2-12, S. plymuthica 3Re4-18, and S. rhizophila DSM14405(T) are promising candidates for further biocontrol strategies. Thus, first steps towards the biological control of Verticillium wilt were presented.
机译:黄萎病是啤酒花中的毁灭性疾病,会造成相当大的农作物损失。 V. albo-atrum是分布最广的物种,而V. dahliae很少发现。由于真菌在静止结构中在土壤中存活数年,并且缺乏有效的化学控制,因此需要一种快速的检测方法。这项研究的目的之一是评估黄萎病菌的发生。在Hallertau地区(德国)。因此,使用实时PCR(聚合酶链反应)分析法分析了76种有或没有枯萎症状的啤酒花植物。在57份血样中鉴定出白纹弧菌。病原体可以偶发出现而没有视觉症状。分析了植物内部真菌的丰度。此外,评估了四种有益细菌,即伯克霍尔德氏菌ZR2-12,假单胞菌RE * 1-1-14,粘质沙雷氏菌3Re4-18和嗜麦单胞菌DSM14405(T),作为啤酒花中的生物防治剂。评估了根系中的定植能力,促进植物生长和拮抗作用。所有菌株都定植于菌毛层和根际。细菌菌株B. terricola ZR2-12,S。plymuthica 3Re4-18和S. rhizophila DSM14405(T)是有望用于进一步生物防治策略的候选菌株。因此,提出了生物防治黄萎病的第一步。

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