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首页> 外文期刊>British Journal of Clinical Pharmacology >Laser Doppler imager (LDI) scanner and intradermal injection for in vivo pharmacology in human skin microcirculation: responses to acetylcholine, endothelin-1 and their repeatability.
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Laser Doppler imager (LDI) scanner and intradermal injection for in vivo pharmacology in human skin microcirculation: responses to acetylcholine, endothelin-1 and their repeatability.

机译：激光多普勒成像仪（LDI）扫描仪和皮内注射用于人体皮肤微循环的体内药理学：对乙酰胆碱，内皮素-1的反应及其可重复性。

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AIMS: The purpose of this study was to evaluate the repeatability of forearm skin blood flow responses to intradermal injections of acetylcholine (ACh) and endothelin-1 (ET-1) using a double injection technique (DIT) and a laser Doppler imager (LDI) scanner in the human skin microcirculation. METHODS: We used a laser Doppler imager (Moor LDI V3.01) to continuously monitor the change in skin blood flow during intradermal administration of physiological saline (0.9% NaCl), acetylcholine (ACh 10(-7), 10(-8), 10(-9) M) and endothelin-1 (ET-1 10(-14), 10(-16), 10(-18) M) in 10 healthy male subjects. Subjects were examined on 3 different days for assessment of interday and interobserver repeatability. Injections of either drug were randomly placed on different sites of the forearm. Laser Doppler images were collected before and after injection at 2.5 min intervals for 30 min. Data were analysed after the completion of each experiment using Moor Software V.3.01. Results are expressed as changes from baseline in arbitrary perfusion units (PU). RESULTS: ACh caused a significant vasodilation (P < 0.0001 anova, mean +/- SE: 766 +/- 152 PU, ACh 10(-9) M; 1868 +/- 360 PU, ACh 10(-8) M; 4188 +/- 848 PU, ACh 10(-7) M; mean of days 1 and 2, n = 10), and ET-1 induced a significant vasoconstrictive response (P < 0.0001 anova, -421 +/- 83 PU, ET-1 10(-18) M; -553 +/- 66 PU, ET-1 10(-16) M; -936 +/- 90 PU, ET-1 10(-14) M; mean of days 1 and 2, n = 10). There was no difference on the response to either drug on repeated days. Bland-Altman analyses showed a close agreement of responses between days with repeatability coefficients of 1625.4 PU for ACh, and 386.0 PU for ET-1 (95% CI: ACh, -1438 to 1747 PU, ET-1, -399 to 358 PU) and between observers with repeatability coefficients of 1057.2 PU for ACh and 255.8 PU for ET-1 (95% CI: ACh, -1024 to 1048 PU, ET-1, -252 to 249 PU). The variability between these responses was independent of average flux values for both ACh and ET-1. There was a significant correlation between responses measured in the same site, in the same individual on two different days by the same observer (ACh, r = 0.94, P < 0.0001; ET-1, r = 0.90, P < 0.0006), and between responses measured by two different observers (ACh, r = 0.94, P < 0.0001; ET-1, r = 0.91, P < 0.0003). CONCLUSION: We have shown that interday and intraobserver responses to intradermal injections of ET-1 and ACh, assessed using the DIT in combination with an LDI scanner, exhibited good reproducibility and may be a useful tool for studying the skin microcirculation in vivo.
机译：目的：这项研究的目的是评估使用双注射技术（DIT）和激光多普勒成像仪（LDI）对皮内注射乙酰胆碱（ACh）和内皮素-1（ET-1）进行皮内注射的前臂皮肤血流反应的可重复性。 ）扫描仪在人体皮肤中的微循环。方法：我们使用激光多普勒成像仪（Moor LDI V3.01）连续监测皮内注射生理盐水（0.9％NaCl），乙酰胆碱（ACh 10（-7），10（-8））时皮肤血流量的变化。 ，10（-9）M）和内皮素1（ET-1 10（-14），10（-16），10（-18）M）。在3个不同的天检查受试者以评估日间和观察者间的可重复性。两种药物的注射均随机放置在前臂的不同部位。在注射前和注射后以2.5分钟的间隔30分钟收集激光多普勒图像。在完成每个实验后，使用Moor Software V.3.01分析数据。结果表示为任意灌注单位（PU）相对于基线的变化。结果：ACh引起显着的血管扩张（P <0.0001方差分析，平均值+/- SE：766 +/- 152 PU，ACh 10（-9）M; 1868 +/- 360 PU，ACh 10（-8）M; 4188 +/- 848 PU，ACh 10（-7）M;第1天和第2天的平均值，n = 10），ET-1诱导了显着的血管收缩反应（P <0.0001 anova，-421 +/- 83 PU，ET -1 10（-18）M; -553 +/- 66 PU，ET-1 10（-16）M; -936 +/- 90 PU，ET-1 10（-14）M;第1天和2，n = 10）。重复几天对两种药物的反应无差异。 Bland-Altman分析显示，各天之间的响应密切相关，ACh的重复系数为1625.4 PU，ET-1的重复系数为386.0 PU（95％CI：ACh，-1438至1747 PU，ET-1，-399至358 PU ）和观察者之间，ACh的重复系数为1057.2 PU，ET-1的重复系数为255.8 PU（95％CI：ACh，-1024至1048 PU，ET-1，-252至249 PU）。这些响应之间的差异与ACh和ET-1的平均通量值无关。同一观察者在两天内不同地点在同一地点，同一个人中测得的响应之间存在显着相关性（ACh，r = 0.94，P <0.0001; ET-1，r = 0.90，P <0.0006），并且两个不同观察者所测得的反应之间的差异（ACh，r = 0.94，P <0.0001; ET-1，r = 0.91，P <0.0003）。结论：我们已经表明，DIT与LDI扫描仪结合使用对皮内注射ET-1和ACh的日间和观察者反应，显示出良好的可重复性，可能是研究体内皮肤微循环的有用工具。

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