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Development of a biomimetic nanoporous membrane for the selective transport of charged proteins

机译:仿生纳米多孔膜的开发,用于选择性转运带电蛋白质

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We report the use of a micrometer-thick platinum-coated nanoporous membrane for the separation of differently charged proteins. A high field strength of about 25 kV m(-1) was applied, using very low transmembrane potentials of +/- 1.5 V between the platinum-coated membranes. The system mimics the cell membrane function of facilitated transport for specific solutes. The selectivity for Lys: BSA: Mb in a mixed protein solution could be tuned readily between the flux ratios of 2: 2: 1 and 96: 1: 12 respectively, by simple variation of the transmembrane potentials from + 1.5 V to -1.5 V. The experimental fluxes agreed closely with calculated fluxes derived from a simple electrophoresis-potential shielding model at favourable transmembrane potentials.
机译:我们报告使用微米厚度的铂涂层纳米多孔膜用于分离带不同电荷的蛋白质。使用镀铂膜之间的+/- 1.5 V的极低跨膜电位,施加约25 kV m(-1)的高场强。该系统模拟了促进特定溶质运输的细胞膜功能。通过简单地将跨膜电势从+ 1.5 V更改为-1.5 V,可以轻松地分别在2:2:1和96:1:12的通量比之间调整混合蛋白溶液中Lys:BSA:Mb的选择性在有利的跨膜电势下,实验通量与从简单的电泳电位屏蔽模型得到的计算通量非常吻合。

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