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首页> 外文期刊>Berliner und Munchener Tierarztliche Wochenschrift >Evaluation of serological tests for detecting tick-borne encephalitis virus (TBEV) antibodies in animals
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Evaluation of serological tests for detecting tick-borne encephalitis virus (TBEV) antibodies in animals

机译：评估动物中tick传脑炎病毒（TBEV）抗体的血清学测试的评估

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摘要

Tick-borne encephalitis (TBE) in animals is not well understood yet. TBE virus (TBEV) serology in several host species could be valuable for epidemiological analyses in the field as well as for the detection of clinical cases. However, performance andsuitability of the available test systems are not well assessed. Therefore, we evaluated two commercial TBEV-ELISA kits in a pilot study and compared them for their suitability in veterinary applications. For this purpose, we tested 163 field collectedgoat sera and evaluated the results by serum neutralization test (SNT) as "gold standard". Twenty-eight SNT positive sera (17.2%) were detected. The best suited ELISA kit was used for determination of a species-specific cutoff for horses, cattle, sheep,goats, pigs, mice, dogs, rabbits and monkeys with defined sera from animals without known or with improbable contact to TBEV. The level of non-specific ELISA results does not only differ between animal species but may also be influenced by the age of thetested animals. The number of sera which tested false positive by ELISA was higher in older than in young sheep. In order to obtain defined polyclonal sera as references, two dogs, cattle, goats, sheep, rabbits and pigs each, as well as one horse and 90mice were immunized four times with a commercially available TBEV vaccine. In conclusion, our results demonstrated that commercial TBEV-ELISA kits are suitable for application in veterinary medicine for both, verification of clinical TBE cases and epidemiological screening. However, positive ELISA results should be verified by SNT. Only a very low number of false negative ELISA-results were found.
机译：动物中的ick传播性脑炎（TBE）尚不完全清楚。几种宿主物种中的TBE病毒（TBEV）血清学对于该领域的流行病学分析以及临床病例的检测可能是有价值的。但是，可用测试系统的性能和适用性尚未得到很好的评估。因此，我们在一项初步研究中评估了两种商业TBEV-ELISA试剂盒，并比较了它们在兽医应用中的适用性。为此，我们测试了163个野外采集的山羊血清，并通过血清中和试验（SNT）评估了结果，以此作为“金标准”。检测到28个SNT阳性血清（17.2％）。最合适的ELISA试剂盒用于确定马，牛，绵羊，山羊，猪，小鼠，狗，兔子和猴子的物种特异性界限值，其血清来自动物，而未知或与TBEV接触不太可能。非特异性ELISA结果的水平不仅在动物物种之间不同，而且还可能受测试动物的年龄的影响。通过ELISA检测到假阳性的血清数量比年长的绵羊高。为了获得确定的多克隆血清作为参考，用市售的TBEV疫苗分别免疫两只狗，牛，山羊，绵羊，兔和猪，以及一匹马和90只小鼠四次。总之，我们的结果表明，商业TBEV-ELISA试剂盒适用于兽医，既可用于临床TBE病例的验证，也可用于流行病学筛查。但是，ELISA阳性结果应通过SNT进行验证。仅发现极少量的假阴性ELISA结果。

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