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首页> 外文期刊>Berliner und Munchener Tierarztliche Wochenschrift >Chemiluminescent immunoassay in comparison with the indirect ELISA as reference method for detecting Salmonella antibodies in swine meat juice
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Chemiluminescent immunoassay in comparison with the indirect ELISA as reference method for detecting Salmonella antibodies in swine meat juice

机译：化学发光免疫测定与间接ELISA的比较作为参考方法检测猪肉汁中沙门氏菌抗体

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摘要

The efficiency of chemiluminescent immunoassay (CLIA) in detecting Salmonella antibodies in the meat juice of slaughter swine was compared with the indirect ELISA (BgVV method). Based on the screening test results of 987 meat juice samples obtained from different laboratories in Germany, a good level of agreement between the two systems was obtained with a kappa value of 0.824 at 20% cut-off and 0.798 at 40% cut-off. At 20% and 40% cut-off levels, a sensitivity of 96.2% and 97.3%, respectively, and a specificity of 94.6% and 95.1%, respectively, were demonstrated between CLIA and ELISA. The detecting LPS antigen was tested for specificity and a cross-reaction with two E. coli and Yersinia strains was found when tested with ELISA. This reaction was not observed in CLIA, possibly because of the broader measurement spectrum of this test which allows a more distinctive definition of immunologic reactions. The same explanation can be given for the increased number of meat juice samples which were positively detected only in ELISA but not in CLIA. The positively classified samples in screening were further tested for reciprocal titers in both test systems, and a higher correlation between screening and titration results was obtained for CLIA. Towards the end of the study, a preliminary comparison, of CLIA with two available commercial ELISA test kits was conducted and the same tendency was observed, namely, wider detection range of CLIA compared to the other tests. Based on the results of this study, CLIA can be used as a reference method in detecting Salmonella antibodies in the meat juice of slaughter pigs.
机译：将化学发光免疫分析法（CLIA）检测出的生猪屠宰肉汁中沙门氏菌抗体的效率与间接ELISA（BgVV方法）进行了比较。根据从德国不同实验室获得的987份肉汁样品的筛选测试结果，两个系统之间的一致性很高，在20％截止时的kappa值为0.824，在40％截止时的kappa值为0.798。在CLIA和ELISA之间，在截止水平为20％和40％时，灵敏度分别为96.2％和97.3％，特异性分别为94.6％和95.1％。测试检测到的LPS抗原的特异性，并通过ELISA测试发现与两种大肠杆菌和耶尔森菌的交叉反应。在CLIA中未观察到此反应，可能是因为该测试的测量范围更广，可以对免疫反应进行更独特的定义。对于仅在ELISA中而不在CLIA中被阳性检测到的肉汁样品数量增加，可以给出相同的解释。筛选中阳性分类的样品在两个测试系统中均进行了相互滴度的进一步测试，并且CLIA的筛选和滴定结果之间具有更高的相关性。在研究即将结束时，对CLIA与两种可用的市售ELISA测试试剂盒进行了初步比较，并观察到了相同的趋势，即与其他测试相比，CLIA的检测范围更广。基于这项研究的结果，CLIA可作为检测屠宰猪肉汁中沙门氏菌抗体的参考方法。

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