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首页> 外文期刊>Berliner und Munchener Tierarztliche Wochenschrift >Comparison of diagnostical methods for detection of an infection with different isolates of infectious haematopoietic necrosis virus (IHNV) [German]
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Comparison of diagnostical methods for detection of an infection with different isolates of infectious haematopoietic necrosis virus (IHNV) [German]

机译：用于检测感染性造血坏死病毒（IHNV）不同分离株感染的诊断方法的比较[德国]

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摘要

The infectious haematopoietic. necrosis. (IHN) is beside the viral haemorrhagic septicemia (VHS) one of the viral fish diseases that have a considerable economic impact on German aquaculture. The measures actually in force are focused on control and spread prevention of the disease. within the borders of the European Union (EU). The detection and confirmation of an outbreak is performed according,to the pertinent EU legislation,which allow the application of methods like the virus neutralisation test (VNT), the immunofluorescence test (IFT) and the enzyme-linked immunosorbent as say (ELISA). Besides the classic virological serblogy methods, further tests for the identification and confirmation of the fish, pathogen like i.e. PCR and DNA probe, techniques are recommended by the OIE. TO. compare diagnostic methods as ELISA, cell cultivation and RT-PCR,rainbow trout of the strain "Isle of Man" were,infected with six IHNV strains. Samples were taken on day, 7 (viraemia period) and at the day 28 of the. trial. The ground organs were inoculated into EPC cells (Epithelioma papulosum Cyprini cells) and examined by ELISA as Well as after RNA extraction by RT-PCR. Besides the determination of the, isolate as well as the virulence for, 20 g trout, significant differences in the demonstration of the viruses were observed. While the RT-PCR demonstrated to be the most sensitive method, antigen ELISA and virus cultivation results showed in dependence of the IHNV isolate that not all viruses were identifiable under the chosen experimental condition in the same manner.
机译：传染性造血。坏死。 （IHN）属于病毒性出血性败血病（VHS）之一，是对德国水产养殖业产生重大经济影响的病毒性鱼类疾病之一。实际采取的措施集中在控制和预防这种疾病。在欧盟（EU）边界内。暴发的检测和确认是根据相关的欧盟法规进行的，该法规允许应用病毒中和测试（VNT），免疫荧光测试（IFT）和酶联免疫吸附剂（ELISA）等方法。 OIE推荐除了经典的病毒学血清学方法外，还建议进一步测试以鉴定和确认鱼类，病原体，例如PCR和DNA探针。至。比较ELISA，细胞培养和RT-PCR等诊断方法，将“ Isle of Man”菌株的虹鳟鱼感染了6株IHNV菌株。在第7天（病毒血症期）和第28天取样。试用。将地面器官接种到EPC细胞（乳头上皮癌细胞）中，通过ELISA以及通过RT-PCR提取RNA后进行检查。除了确定20 g鳟鱼的分离株和毒力外，还观察到了病毒展示的显着差异。尽管RT-PCR被证明是最灵敏的方法，但是抗原ELISA和病毒培养结果表明，依赖于IHNV分离株，并非在选择的实验条件下以相同的方式可识别所有病毒。

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