Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis.

Bao W; Sheldon PJ; Hutchinson CR

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Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis.

机译：peucetius peucetius链霉菌DpsCβ-酮酰基：酰基载体蛋白合酶III的纯化和性质，该酶指定II型聚酮化合物生物合成的丙酸酯-起始剂单元。

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Biosynthesis of the polyketide-derived carbon skeleton of daunorubicin (DNR) begins with propionate rather than acetate, which is the starter unit for most other aromatic polyketides. The dpsCgene has been implicated in specifying the unique propionate-starter unit, and it encodes a protein that is very similar to the Escherichia coli beta-ketoacyl:acyl carrier protein (ACP) synthase III (FabH or KS III) enzyme of fatty acid biosynthesis. Purified DpsC was found to use propionyl-coenzyme A as substrate and to be acylated by propionate at the Ser-118 residue. DpsC exhibits KS III activity in catalyzing the condensation of propionyl-CoA and malonyl-ACP, and also functions as an acyltransferase in the transfer of propionate to an ACP. The DpsC enzyme has a high-substrate specificity, utilizing only propionyl-CoA, and not malonyl-CoA, 2-methylmalonyl-CoA or acetyl-CoA, as the starter unit of DNR biosynthesis.

机译：柔红霉素（DNR）的聚酮化合物衍生碳骨架的生物合成始于丙酸酯而不是乙酸酯，这是大多数其他芳香族聚酮化合物的起始单元。 dpsC基因已牵涉到指定独特的丙酸酯-启动子单元，并且其编码的蛋白质与脂肪酸生物合成的大肠杆菌β-酮酰基：酰基载体蛋白（ACP）合酶III（FabH或KS III）酶非常相似。发现纯化的DpsC使用丙酰基辅酶A作为底物，并在Ser-118残基处被丙酸酯酰化。 DpsC在催化丙酰辅酶A和丙二酰ACP的缩合反应中表现出KS III活性，并且在丙酸酯向ACP的转移中也起酰基转移酶的作用。 DpsC酶具有高底物特异性，仅利用丙酰基-CoA，而不利用丙二酰基-CoA，2-甲基丙二酰基-CoA或乙酰基-CoA作为DNR生物合成的起始单元。

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《Biochemistry》 |1999年第30期|共6页
作者
Bao W; Sheldon PJ; Hutchinson CR;
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正文语种 eng
中图分类生物化学;
关键词
Multienzyme Complexes; Streptomyces; 3 Oxoacyl Acyl Carrier Protein Synthase chemistry; 多酶复合物; 链霉菌属;

机译：Multienzyme Complexes;Streptomyces;3 Oxoacyl Acyl Carrier Protein Synthase chemistry;多酶复合物;链霉菌属;

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1. Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis. [J] . Bao W, Sheldon PJ, Hutchinson CR Biochemistry . 1999,第30期

机译：peucetius peucetius链霉菌DpsCβ-酮酰基：酰基载体蛋白合酶III的纯化和性质，该酶指定II型聚酮化合物生物合成的丙酸酯-起始剂单元。
2. Functional characterization of the acyl carrier protein (PfACP) and beta-ketoacyl ACP synthase III (PfKASIII) from Plasmodium falciparum. [J] . Waters N, Kopydlowski K, Guszczynski T, Molecular and Biochemical Parasitology . 2002,第2期

机译：恶性疟原虫的酰基载体蛋白（PfACP）和β-酮酰基ACP合酶III（PfKASIII）的功能表征。
3. Acylation of Streptomyces type II polyketide synthase acyl carrier proteins [J] . Bibb Maureen J, Byrom Kate J, Cox Russell J, FEBS Letters . 1998,第1a2期

机译：链霉菌II型聚酮合酶酰基载体蛋白的酰化作用
4. Polyketide Biosynthesis beyond the Type I, II, and IIIPolyketide Synthase Paradigms: A Progress Report [C] . Ben Shen, Yi-Qiang Cheng, Steven D. Christenson, American Chemical Society . 2006

机译：聚酮化合物生物合成超出I型，II和IIIPolykete Synthase Paradigms：进度报告
5. Discovery of novel inhibitors of beta-ketoacyl-acyl carrier protein synthase III (FabH). [D] . He, Xin. 2002

机译：发现了β-酮酰基-酰基载体蛋白合酶III（FabH）的新型抑制剂。
6. Functional analysis of putative beta-ketoacyl:acyl carrier protein synthase and acyltransferase active site motifs in a type II polyketide synthase of Streptomyces glaucescens. [O] . G Meurer, C R Hutchinson 1995

机译：褐链霉菌II型聚酮合酶中推定的β-酮酰基：酰基载体蛋白合酶和酰基转移酶活性位点基序的功能分析。
7. Functional analysis of putative beta-ketoacyl:acyl carrier protein synthase and acyltransferase active site motifs in a type II polyketide synthase of Streptomyces glaucescens. [O] . Meurer, G, Hutchinson, C R 1995

机译：褐链霉菌II型聚酮合酶中推定的β-酮酰基：酰基载体蛋白合酶和酰基转移酶活性位点基序的功能分析。

Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis.

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