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首页> 外文期刊>Graefe's archive for clinical and experimental ophthalmology: Albrecht von Graefes Archiv fur klinische und experimentelle Opthalmologie >The contrasting effect of estrogen on mRNA expression of VEGF in bovine retinal vascular endothelial cells under different oxygen conditions.
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The contrasting effect of estrogen on mRNA expression of VEGF in bovine retinal vascular endothelial cells under different oxygen conditions.

机译：不同氧条件下雌激素对牛视网膜血管内皮细胞VEGF mRNA表达的影响

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OBJECTIVE: To investigate the influence of Estradial (E(2)) at physiological concentration on mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1alpha(HIF-alpha1) in bovine retinal vascular endothelial cells (BRECs) under different oxygen conditions. METHODS: The mRNA expression of VEGF, HIF-1alpha in BRECs was studied by quantitative reverse-transcription PCR (qRT-PCR), and protein levels were assayed by Western Blot. Different concentrations of E(2) (10(-12), 10(-10), 10(-8) mol/l) and estrogen receptor antagonist Tamoxifen (10(-7) mol/l) were added into the cell culture medium of different groups, while the phosphate-buffered saline (PBS) was added in the control group instead. Culture conditions were set to be under normoxia and hypoxia. The results of each group were detected after 8 hours and 24 hours. RESULTS: (1) Under hypoxia, gene expression of VEGF, HIF-1alpha in BRECs increased more than that of the control group (P < 0.05). There is evident positive correlation between the mRNA and protein levels of VEGF and HIF-1alpha (r = 0.82). (2) Treated with 10(-8) mol/l E(2,) the expression of VEGF mRNA was increased in a time-dependent manner (P < 0.05). Treated with the indicated to concentrations of E(2) (10(-12 approximately -8) mol/l) under normoxia for twenty-four hours, the mRNA expression of VEGF in BRECs was increased in a dose-dependent manner (P < 0.05), whereas E(2) did not influence the mRNA expression of HIF-1alpha (P > 0.05). (3) Under hypoxia , E(2) reduced the mRNA and protein levels of HIF-1alpha and VEGF in a concentration-dependent manner (P < 0.05), and the decrease developed in a time-dependent manner (P < 0.05). (4) An overdose of tamoxifen (10(-7) mol/l) reversed the effect of E(2) (10(-8) mol/l) (P < 0.05). CONCLUSIONS: E(2) increases the gene expression of VEGF in BRECs under normoxia, whereas E(2) reduces the gene expression of VEGF in BRECs through HIF-1alpha under hypoxic conditions in a dose-dependent and time-dependent manner. The contrasting effect of E(2) on mRNA expression of VEGF may play a prophylactic role in retinopathy of prematurity (ROP).
机译：目的：研究生理浓度下的雌二醇（E（2））对牛视网膜血管内皮细胞（BRECs）下血管内皮生长因子（VEGF）和缺氧诱导因子-1α（HIF-alpha1）mRNA表达的影响。不同的氧气条件。方法：采用定量逆转录PCR（qRT-PCR）研究BRECs中VEGF，HIF-1α的mRNA表达，并通过Western Blot检测蛋白水平。将不同浓度的E（2）（10（-12），10（-10），10（-8）mol / l）和雌激素受体拮抗剂他莫昔芬（10（-7）mol / l）添加到细胞培养物中在对照组中加入磷酸盐缓冲盐水（PBS）。培养条件设定为常氧和低氧。在8小时和24小时后分别检测每组的结果。结果：（1）在低氧条件下，BRECs中VEGF，HIF-1α的基因表达增高，高于对照组（P <0.05）。 VEGF和HIF-1alpha的mRNA和蛋白水平之间存在明显的正相关（r = 0.82）。 （2）10（-8）mol / l E（2，）处理后，VEGF mRNA的表达呈时间依赖性（P <0.05）。在常氧下用指示浓度的E（2）（10（-12大约-8）mol / l）处理24小时，BRECs中VEGF的mRNA表达呈剂量依赖性增加（P < 0.05），而E（2）不会影响HIF-1alpha的mRNA表达（P> 0.05）。 （3）在缺氧条件下，E（2）以浓度依赖的方式降低了HIF-1alpha和VEGF的mRNA和蛋白水平（P <0.05），并且以时间依赖的方式降低了PIF（0.05）。 （4）过量的他莫昔芬（10（-7）mol / l）逆转了E（2）（10（-8）mol / l）的作用（P <0.05）。结论：E（2）在常氧下增加BRECs中VEGF的基因表达，而E（2）在低氧条件下通过HIF-1alpha降低BRECs中VEGF的基因表达，且呈剂量依赖性和时间依赖性。 E（2）对VEGF mRNA表达的对比作用可能在早产儿视网膜病变（ROP）中起到预防作用。

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