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首页> 外文期刊>Early human development >Gender-related effects of prenatal administration of estrogen and progesterone receptor antagonists on VEGF and surfactant-proteins and on alveolarisation in the developing piglet lung.
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Gender-related effects of prenatal administration of estrogen and progesterone receptor antagonists on VEGF and surfactant-proteins and on alveolarisation in the developing piglet lung.

机译：产前给予雌激素和孕激素受体拮抗剂对发育中的仔猪肺中VEGF和表面活性剂蛋白以及肺泡形成的性别相关影响。

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BACKGROUND: Vascular endothelial growth factor (VEGF) is essential for embryonic lung development and has been shown to be regulated by estradiol (E2) and progesterone (P). AIM: To investigate the effects of prenatal E2 and P withdrawal by specific receptor antagonists on the mRNA expression of VEGF, surfactant proteins (SP-B and SP-C) and on alveolarisation in lung tissue of male and female pig fetuses. METHODS: Fetuses from 10 sows were randomized to receive either both an intramuscular injection of the E2 receptor blocker ICI 182.780 and the P receptor blocker RTI 3021-022 (ICI+RTI, n=5) or a placebo injection (n=5) at 90 days of gestation (DOG, 115=term). After delivery by cesarean section on 114 DOG, tissue of the left lingula of the piglet's lung (28 placebo, 26 ICI+RTI) was obtained to determine the mRNA expression of VEGF, SP-B and SP-C. Lungs from 15 placebo and 14 ICI+RTI group piglets were removed and alveolar counts performed. RESULTS: The ICI+RTI group showed significantly lower SP-C mRNA expression and alveolar counts compared to the placebo group (p=0.04 and 0.03, respectively). Diminished alveolarisation in the ICI+RTI group was mainly due to the reduction of alveolar counts in male piglets (p=0.02). Within the placebo group VEGF and SP-B mRNA expression in male piglets were significantly lower compared to female piglets (p=0.01 and 0.004, respectively). ICI+RTI treatment abolished this gender-related difference. CONCLUSION: Estradiol and P antagonism affected gender-related differences of key proteins for pulmonary function and development and especially in males was associated with diminished alveolarisation.
机译：背景：血管内皮生长因子（VEGF）对于胚胎肺发育至关重要，并且已显示受雌二醇（E2）和孕酮（P）调节。目的：研究特异性受体拮抗剂对产前E2和P的撤回对男女猪胎儿肺组织VEGF，表面活性剂蛋白（SP-B和SP-C）mRNA表达和肺泡形成的影响。方法：将10头母猪的胎儿随机接受肌肉注射E2受体阻滞剂ICI 182.780和P受体阻滞剂RTI 3021-022（ICI + RTI，n = 5）或安慰剂注射（n = 5）。妊娠90天（DOG，115 =足月）。在114 DOG剖宫产后，获得仔猪肺左舌舌的组织（28个安慰剂，26 ICI + RTI），以测定VEGF，SP-B和SP-C的mRNA表达。从15只安慰剂和14只ICI + RTI组仔猪中取出肺，进行肺泡计数。结果：与安慰剂组相比，ICI + RTI组的SP-C mRNA表达和肺泡计数显着降低（分别为p = 0.04和0.03）。 ICI + RTI组的肺泡分泌减少主要是由于雄性仔猪的肺泡计数减少（p = 0.02）。在安慰剂组内，雄性仔猪的VEGF和SP-B mRNA表达明显低于雌性仔猪（分别为p = 0.01和0.004）。 ICI + RTI治疗消除了这种性别相关差异。结论：雌二醇和P拮抗作用影响了与性别有关的肺功能和发育关键蛋白质的差异，尤其是男性，这与肺泡减少有关。

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