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Different digestion enzymes used for human pancreatic islet isolation: A mixed treatment comparison (MTC) meta-analysis

机译：用于人类胰岛分离的不同消化酶：混合治疗比较（MTC）荟萃分析

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Collagenases are critical reagents determining yield and quality of isolated human pancreatic islets and may affect islet transplantation outcome. Some islet transplantation centers have compared 2 or more collagenase blends; however, the results regarding differences in quantity and quality of islets are conflicting. Thus, for the first time, a mixed treatment comparison (MTC) meta-analysis was carried out to compile data about the effect of different collagenases used for human pancreas digestion on islet yield, purity, viability and stimulation index (SI). Pubmed, Embase and Cochrane libraries were searched. Of 755 articles retrieved, a total of 15 articles fulfilled the eligibility criteria and were included in the MTC meta-analysis. Our results revealed that Vitacyte and Liberase MTF were associated with a small increase in islet yield (islet equivalent number/g pancreas) when compared with Sevac enzyme [standardized mean difference (95% credible interval - CrI) = -2.19 (-4.25 to -0.21) and -2.28 (-4.49 to -0.23), respectively]. However, all other enzyme comparisons did not show any significant difference regarding islet yield. Purity and viability percentages were not significantly different among any of the analyzed digestion enzymes. Interestingly, Vitacyte and Serva NB1 were associated with increased SI when compared with Liberase MTF enzyme [unstandardized weighted mean difference (95% CrI) = -1.69 (-2.87 to -0.51) and -1.07 (-1.79 to -0.39), respectively]. In conclusion, our MTC meta-analysis suggests that the digestion enzymes currently being used for islet isolation works with similar efficiency regarding islet yield, purity and viability; however, Vitacyte and Serva NB1 enzymes seem to be associated with an improved SI as compared with Liberase MTF.
机译：胶原蛋白是决定分离的人胰岛的产量和质量的关键试剂，可能会影响胰岛移植的结果。一些胰岛移植中心比较了2种或更多的胶原酶混合物。但是，关于胰岛数量和质量差异的结果却是矛盾的。因此，首次进行了混合处理比较（MTC）荟萃分析，以汇编有关用于人类胰腺消化的不同胶原酶对胰岛产量，纯度，生存力和刺激指数（SI）的影响的数据。搜索Pubmed，Embase和Cochrane库。在检索到的755篇文章中，共有15篇符合资格标准的文章被纳入了MTC荟萃分析。我们的结果显示，与Sevac酶相比，Vitacyte和Liberase MTF与胰岛产量的小幅增加（胰岛当量数/ g胰腺）相关[标准平均差异（95％可信区间-CrI）= -2.19（-4.25至- 0.21）和-2.28（-4.49至-0.23）]。但是，所有其他酶比较均未显示出关于胰岛产量的任何显着差异。在所分析的任何消化酶之间，纯度和生存力百分比均无显着差异。有趣的是，与Liberase MTF酶相比，Vitacyte和Serva NB1与SI升高有关[未标准化的加权平均差异（95％CrI）分别为-1.69（-2.87至-0.51）和-1.07（-1.79至-0.39）] 。总而言之，我们的MTC荟萃分析表明，目前用于胰岛分离的消化酶在胰岛产量，纯度和生存力方面具有相似的效率。然而，与Liberase MTF相比，Vitacyte和Serva NB1酶似乎与SI改善有关。

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