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Immunocytochemical staining for islet amyloid polypeptide in pancreatic endocrine tumors

机译：胰内分泌肿瘤胰岛淀粉样多肽的免疫细胞化学染色

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Aims/hypothesis: Islet amyloid polypeptide is originally identified as the chief constituent of amyloid in insulinomas and type 2 diabetic islets. This study aimed to identify islet amyloid polypeptide by immunocytochemical staining in pancreatic endocrine tumors including 30 cases of insulinomas and non-β-cell pancreatic endocrine tumors. Results: In normal islets, 62% of islet cells and 52% of insulin cells were granularly positive for insulin and IAPP, respectively, with more insulin positive cells than IAPP positive cells and some densely positive staining for insulin and IAPP in irregularly shaped a nuclear, degenerating islet β-cells. In pancreatic endocrine tumors, all 10 insulinomas were positive for islet amyloid polypeptide but 2 glucogonomas, 1 somatostatinoma, 6 of 7 pancreatic polypeptidomas, all 7 gastrinomas and all 3 non-functioning pancreatic endocrine tumors were negative for islet amyloid polypeptide whereas one pancreatic polypeptidoma was positive for islet amyloid polypeptide. Methods: Using commercially available rabbit anti-islet amyloid polypeptide antibody, immunocytochemical staining was performed on 30 cases of pancreatic endocrine tumors, consisting of 10 insulinomas, 2 glucagonomas, 1 somatostatinoma, 7 pancreatic polypeptidomas, 7 gastrinomas and 3 non-functioning pancreatic endocrine tumors. Pancreatic tissues containing pancreatic endocrine tumors were systematically immunostained for insulin, glucagon, somatostatin, pancreatic polypeptide, gastrin and chromogranin A, in addition to islet amyloid polypeptide. When normal pancreatic tissues adjacent to pancreatic endocrine tumors were present, insulin, glucagon, somatostatin and islet amyloid polypeptide positive cells were counted for a total of 20 islets, which were divided into large islets and medium islets for each case. Conclusions/Interpretations: All 10 insulinomas and 1 pancreatic polypeptidoma were granularly positive for islet amyloid polypeptide, suggesting all 10 insulinomas contained enough insulin granules for IAPP whereas only one non-β-cell pancreatic endocrine tumor was co-localized with islet amyloid polypeptide in their secretary granules.
机译：目的/假设：胰岛淀粉样蛋白多肽最初被认为是胰岛素瘤和2型糖尿病胰岛中淀粉样蛋白的主要成分。本研究旨在通过免疫细胞化学染色在胰内分泌肿瘤中鉴定胰岛淀粉样多肽，包括30例胰岛素瘤和非β细胞胰腺内分泌肿瘤。结果：在正常的胰岛中，胰岛和IAPP的颗粒细胞分别为62％和52％呈颗粒阳性，胰岛素阳性细胞比IAPP阳性细胞多，并且在不规则形状的核中对胰岛素和IAPP的染色浓密，退化的胰岛β细胞。在胰腺内分泌肿瘤中，所有10个胰岛素瘤的胰岛淀粉样多肽均为阳性，但2个糖腺癌，1个生长抑素瘤，7个胰腺多肽瘤中的6个，所有7个胃泌素瘤和所有3个无功能的胰腺内分泌肿瘤对胰岛淀粉样多肽均为阴性，而一个胰多肽肽瘤为阴性。胰岛淀粉样多肽阳性。方法：使用市售的兔抗胰岛淀粉样多肽抗体，对30例胰腺内分泌肿瘤进行免疫细胞化学染色，包括10个胰岛素瘤，2个胰高血糖素瘤，1个生长抑素瘤，7个胰多肽肽瘤，7个胃泌素瘤和3个无功能的胰腺内分泌肿瘤。除胰岛淀粉样蛋白多肽外，对含有胰腺内分泌肿瘤的胰腺组织进行了系统的胰岛素，胰高血糖素，生长抑素，胰腺多肽，胃泌素和嗜铬粒蛋白A免疫染色。当存在与胰腺内分泌肿瘤相邻的正常胰腺组织时，计数胰岛素，胰高血糖素，生长抑素和胰岛淀粉样多肽阳性细胞的总数为20个胰岛，每种情况分为大胰岛和中胰岛。结论/解释：所有10个胰岛素瘤和1个胰多肽瘤颗粒均呈胰岛淀粉样多肽阳性，表明所有10个胰岛素瘤均含有足够用于IAPP的胰岛素颗粒，而只有1个非β细胞胰腺内分泌肿瘤与胰岛淀粉样多肽共定位。秘书颗粒。

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