• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Islets >Histopathology and ex vivo insulin secretion of pancreatic islets in gestational diabetes: A case report
【24h】

Histopathology and ex vivo insulin secretion of pancreatic islets in gestational diabetes: A case report

机译：妊娠糖尿病胰岛的组织病理学和离体胰岛素分泌：一例报告

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gestational diabetes (GD) results from insufficient endogenous insulin supply. No information is available on features of islet cells in human GD. Herein, we describe several properties of islets from a woman with GD. Immunohistochemical stainings and EM analyses were performed on pancreatic samples. Islet isolation was achieved by enzymatic dissociation and density gradient centrifugation. Ex vivo insulin secretion was studied in response to fuel secretagogues. Control islets were obtained from matched non-pregnant, non-diabetic women. Total insulin positive area was lower in GD, mainly due to the presence of smaller islets. β-cell apoptosis and the presence of Ki67 positive islet cells were similar in GD and controls, whereas the amount of insulin positive cells in or close to the ducts was decreased in GD. Ex vivo insulin secretion did not differ between GD and non-pregnant, non-diabetic islets. These findings suggest that in this case of human GD there might mainly be a defect of β-cell amount, not due to increased apoptosis, but possibly to insufficient regeneration.
机译：妊娠糖尿病（GD）是由于内源性胰岛素供应不足引起的。没有有关人GD中胰岛细胞特征的信息。在本文中，我们描述了患有GD的女性的胰岛的几种特性。对胰腺样品进行免疫组织化学染色和EM分析。通过酶离解和密度梯度离心分离胰岛。针对燃料促分泌素的反应，研究了离体胰岛素分泌。从匹配的非妊娠，非糖尿病妇女中获得对照胰岛。 GD总胰岛素阳性面积较低，主要是由于存在较小的胰岛。 GD和对照组中的β细胞凋亡和Ki67阳性胰岛细胞的存在相似，而GD中或附近的胰岛素阳性细胞的数量减少。 GD和非妊娠，非糖尿病胰岛之间的离体胰岛素分泌没有差异。这些发现表明，在这种情况下，人GD可能主要是β细胞数量的缺陷，而不是由于细胞凋亡增加，而是由于再生不足。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号