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首页> 外文期刊>Glia >Transcriptional Profiling Predicts Overwhelming Homology of Schwann Cells, Olfactory Ensheathing Cells, and Schwann Cell-Like Glia
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Transcriptional Profiling Predicts Overwhelming Homology of Schwann Cells, Olfactory Ensheathing Cells, and Schwann Cell-Like Glia

机译：转录分析预测雪旺细胞，嗅鞘细胞和雪旺细胞样胶质细胞的压倒性同源。

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Schwann cells (SCs), olfactory ensheathing cells (OECs), and central nervous system Schwann cell-like glia (SG) represent a group of nerve growth factor receptor p75 (NGFR)-positive cells, originating from different tissues. Because of their proregenerative capacities, these cells are subjects in experimental transplantation-based therapies of spinal cord trauma. The objective of this study was to compare the transcriptomes of uninfected and canine distemper virus-infected OECs, SCs, SG and fibroblasts (FBs) derived from four beagle dogs and cultured under identical conditions in vitro, employing canine genome 2.0 arrays (Affymetrix). Here, we observed a complete lack of transcriptional differerences between OECs and SG, a high similarity of OECs/SG to SCs, and a marked difference of SCs and OECs/SG towards FBs. Differentially expressed genes possibly involved in the maintenance of cell type-specific identity included an up-regulation of HOXD8 and HOXC4 in SCs, and an up-regulation of CNTNAP2 and EFEMP1 in OECs/SG. We identified cell type-specific biomarkers employing supervised clustering with a K-nearest-neighbors algorithm and correlation-based feature selection. Thereby AQP1 and SCRG1 were predicted to be the most powerful biomarkers distinguishing SCs from OECs/SG. Immunofluorescence confirmed a higher expression of SCRG1 in OECs and SG, and conversely a higher expression of AQP1 in SCs in vitro. Furthermore, canine and murine olfactory nerves showed SCRG1-positive, AQP1-negative OECs and/or axons, whereas sciatic nerves displayed multifocal non-myelinated, AQP1-positive, SCRG1-negative cells. Conclusively, OECs/SG are suggested to be a uniform cell type differing only in the tissue of origin and highly related to SCs.
机译：雪旺氏细胞（SCs），嗅鞘细胞（OEC）和中枢神经系统雪旺氏细胞样神经胶质细胞（SG）代表一组神经生长因子受体p75（NGFR）阳性细胞，起源于不同组织。由于它们的再生能力，这些细胞成为基于实验性移植的脊髓损伤治疗的对象。这项研究的目的是比较未感染和犬瘟热病毒感染的OEC，SC，SG和成纤维细胞（FB）的转录组，这些转录组来自四只比格犬，并在相同条件下体外培养，采用犬基因组2.0阵列（Affymetrix）。在这里，我们观察到OEC和SG之间完全没有转录差异，OEC / SG与SC的高度相似，以及SC和OEC / SG与FB的显着差异。可能参与维持细胞类型特异性同一性的差异表达基因包括SC中HOXD8和HOXC4的上调，以及OECs / SG中CNTNAP2和EFEMP1的上调。我们使用K-最近邻算法和基于相关性的特征选择，通过监督聚类确定了特定细胞类型的生物标记。因此，AQP1和SCRG1被认为是区分SC与OEC / SG的最有力的生物标记。免疫荧光实验证实，OEC和SG中SCRG1的表达较高，而体外SC中AQP1的表达较高。此外，犬和鼠嗅神经显示出SCRG1阳性，AQP1阴性的OEC和/或轴突，而坐骨神经则显示了多灶性非髓鞘，AQP1阳性，SCRG1阴性的细胞。结论是，建议OEC / SG是一种统一的细胞类型，仅在起源组织方面不同，并且与SC高度相关。

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