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首页> 外文期刊>FEMS Microbiology Ecology >Effects of soil improvement treatments on bacterial community structure and soil processes in an upland grassland soil
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Effects of soil improvement treatments on bacterial community structure and soil processes in an upland grassland soil

机译：土壤改良剂对旱地草原土壤细菌群落结构和土壤过程的影响

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Temporal temperature gradient electrophoresis (TTGE) analysis of 16S rRNA gene fragments amplified with primers selective for eubacteria and beta-proteobacterial ammonia-oxidising bacteria (AOB) was used to analyse changes in bacterial and AOB community profiles of an upland pasture following soil improvement treatments (addition of sewage sludge and/or lime). Community structure was compared with changes in activity assessed by laboratory measurements of basal respiration and ammonia oxidation potentials, and with measurements of treatment- and time-related changes in soil characteristics. The predominant bacterial populations had a high degree of similarity under all treatment regimens, which was most pronounced early in the growing season. Most of the differences that occurred between soil samples with time could be accounted for by spatial and temporal variation; however, analysis of variance and cluster analysis of similarities between 16S rDNA TTGE profiles indicated that soil improvement treatments exerted some effect on community structure. Lime application had the greatest influence. The impact of soil improvement treatments on autotrophic ammonia oxidation was significant and sustained, especially in soils which had received sewage sludge and lime treatments in combination. However, despite obvious changes in soil characteristics, e.g. pH and soil nitrogen, increasing heterogeneity in the AOB community structure over time obscured the treatment effects observed at the beginning of the experiment. Nevertheless, time series analysis of AOB TTGE profiles indicated that the AOB community in improved soils was more dynamic than in control soils where populations were found to be relatively stable. These observations suggest that the AOB populations exhibited a degree of functional redundancy. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. [References: 37]
机译：使用对真细菌和β-变形细菌氨氧化细菌（AOB）有选择性的引物扩增的16S rRNA基因片段的时间温度梯度电泳（TTGE）分析，用于分析土壤改良处理后旱地牧场细菌和AOB群落特征的变化（添加污水污泥和/或石灰）。将群落结构与通过实验室测量基础呼吸和氨氧化电位评估的活动变化，以及与处理和时间相关的土壤特征变化进行评估相比较。在所有处理方案下，主要细菌种群具有高度相似性，这在生长期初期最为明显。土壤样品之间随时间变化的大多数差异可以通过时空变化来解释。然而，16S rDNA TTGE图谱之间的相似性的方差分析和聚类分析表明，土壤改良处理对群落结构产生了一定影响。石灰的施用影响最大。土壤改良处理对自养氨氧化的影响是显着且持续的，尤其是在已接受污水污泥和石灰处理的土壤中。然而，尽管土壤特性发生了明显变化，例如pH和土壤氮，随着时间的推移AOB群落结构异质性的增加，掩盖了实验开始时观察到的处理效果。但是，对AOB TTGE分布图进行时间序列分析表明，改良土壤中的AOB群落比对照土壤中的种群动态更为稳定，而对照土壤中的AOB群落则更为动态。这些观察结果表明，AOB种群表现出一定程度的功能冗余。 （C）2003年欧洲微生物学会联合会。由Elsevier B.V.发布。保留所有权利。 [参考：37]

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