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首页> 外文期刊>FEMS Microbiology Ecology >Morphology, genetic diversity, temperature tolerance and toxicity of Cylindrospermopsis raciborskii (nostocales, cyanobacteria) strains from Thailand and Japan
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Morphology, genetic diversity, temperature tolerance and toxicity of Cylindrospermopsis raciborskii (nostocales, cyanobacteria) strains from Thailand and Japan

机译：来自泰国和日本的拟青冈拟南芥（Nostocales，cyanobacteria）菌株的形态，遗传多样性，耐温性和毒性

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摘要

Cylinderospermopsis raciborskii is a planktonic, nostocalean cyanobacterium, which produces an alkaloid heptatoxin, cylindro-spermopsin. We performed morphological observations, 16S rDNA sequence analysis, PCR fingerprint analysis of short tandemly repeated repetitive (STRR) sequences, temperature tolerances and toxin analysis to characterize 24 strains of this toxic cyanobacterium isolated from Thailand and Japan. All strains shared common morphological traits characteristic of C raciborskii and showed high 16S rDNA sequence similarity, forming a defined cluster together with the reference strains from Australia. In particular, some of the Thai strains shared 99.9% to 100% similarity with the Australian strains. Various combinations of STRR primers revealed different and unique DNA band patterns among strains of C raciborskii. The phylogenetic tree revealed two main Clusters of C raciborskii strains, the Thai/Japan-Shinobazugaike Cluster (cluster 1) and the Japan-Gonoike cluster (cluster II). Cluster I was further divided into two subclusters, A (only Thai strains) and B (one Thai strain and the Japan-Shinobazugaike strains). Thus, the results from 16S rDNA and STRR analyses showed no clear geographical distinction between Japanese and Thai strains and between Thai and Australian strains. Thai strains were separated into adaptive and non-adaptive groups to low temperature (15 and 17.5 degreesC) and Japanese strains were composed of only low-temperature-adaptive ones. The toxin cylindrospermopsin was detected in some strains of Cluster I-A and in one strain of cluster II. We conclude that C raciborskii is a species that has recently begun to invade, and a species with different physiological strains or ecotypes in temperature tolerance; the toxin is synthesized without any relation to phylogenetic or genetic Clusters and to geography. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
机译：Cylinderospermopsis raciborskii是一种浮游的，能携带生物碱的蓝藻，能产生生物碱庚毒素，cylindro-spermopsin。我们进行了形态学观察，16S rDNA序列分析，短串联重复序列（STRR）序列的PCR指纹分析，温度耐受性和毒素分析，以表征从泰国和日本分离出的24株这种有毒蓝细菌的特征。所有菌株均具有共同的形态特征，并具有很高的16S rDNA序列相似性，与澳大利亚的参考菌株一起形成了明确的簇。特别是，一些泰国菌株与澳大利亚菌株具有99.9％至100％的相似性。 STRR引物的各种组合显示了拉氏梭菌菌株之间不同且独特的DNA条带模式。系统发育树显示了两个主要的拉克伯氏菌菌株，泰国/日本-Shinobazugaike簇（第1类）和Japan-Gonoike簇（第II类）。群集I进一步分为两个子类，即A（仅泰国菌株）和B（一个泰国菌株以及Japan-Shinobazugaike菌株）。因此，来自16S rDNA和STRR分析的结果表明，日本和泰国菌株之间以及泰国和澳大利亚菌株之间没有明确的地理区别。泰国菌株被分为低温和低温的适应组和非适应组（15和17.5摄氏度），日本菌株仅由低温适应组组成。在一些群集I-A的菌株和一个群集II的菌株中检测到了cylindrospermopsin毒素。我们得出的结论是，中华绒螯蟹是最近才开始入侵的物种，并且其耐温性具有不同的生理菌株或生态型。该毒素的合成与系统发育或遗传簇以及地理无关。 （C）2004年欧洲微生物学会联合会。由Elsevier B.V.发布。保留所有权利。

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