...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEMS Microbiology Letters >A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes
【24h】

A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes

机译：螺旋藻在整体差异表达蛋白，脂肪酸生物合成基因的mRNA水平和稳定性方面的组合胁迫响应分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional- differential gel electrophoresis was then performed to separate differentially expressed proteins that were subsequently identified by MS. Among all differentiated proteins identified, a protein involved in fatty acid biosynthesis, (3R)-hydroxymyristoyl-[acyl-carrier-protein]-dehydratase encoded by fabZ, was the most up-regulated protein. However, the fatty-acid desaturation proteins were not significantly differentiated. This raised the question of how the unsaturated fatty acid, especially gamma-linolenic acid, content in the cells in the cold-dark shift remained stable compared with that of the cold shift. Thus, a study at the transcriptional level of these desaturase genes, desC, desA and desD, and also of the fabZ gene was conducted. The results indicated that in the dark, where energy is limited, mRNA stability was enhanced by exposure to low temperatures. The data demonstrate that when the cells encounter cold stress with energy limitation, they can maintain their homeoviscous adaptation ability via mRNA stability.
机译：基因表达的变化在增强蓝细菌在寒冷条件下生存的能力中起着至关重要的作用。在本研究中，螺旋藻培养物在最佳生长温度下于光照下生长，然后转移至22°C的黑暗条件下。然后进行二维差分凝胶电泳以分离差异表达的蛋白质，随后通过多发性硬化症。在鉴定出的所有分化蛋白中，由fabZ编码的与脂肪酸生物合成有关的蛋白（3R）-羟基肉豆蔻酰基-[酰基载体蛋白]脱水酶是上调最多的蛋白。但是，脂肪酸去饱和蛋白没有明显的分化。这就提出了一个问题，即冷暗转变时细胞中的不饱和脂肪酸，尤其是γ-亚麻酸与冷转变相比如何保持稳定。因此，在这些去饱和酶基因desC，desA和desD以及fabZ基因的转录水平上进行了研究。结果表明，在黑暗中，能量受到限制，暴露于低温下可增强mRNA的稳定性。数据表明，当细胞遇到受能量限制的冷应激时，它们可以通过mRNA稳定性维持其自身的粘膜适应能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号