An endo-β-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans

B?hle L.A.; Mathiesen G.; Vaaje-Kolstad G.; Eijsink V.G.

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An endo-β-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans

机译：粪肠球菌V583的内切β-N-乙酰氨基葡萄糖苷酶，负责水解高甘露糖和杂合型N-连接聚糖

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It has been demonstrated previously that Enterococcus faecalis produces secreted endoglycosidases that enable the bacteria to remove N-linked glycans from glycoproteins. One enzyme potentially responsible for this activity is EF0114, comprising a typical GH18 endoglycosidase domain and a GH20 domain. We have analyzed the other candidate, EF2863, and show that this predicted single domain GH18 protein is an endo-β-N-acetylglucosaminidase. EF2863 hydrolyzes the glycosidic bond between two N-acetylglucosamines (GlcNAc) in N-linked glycans of the high-mannose and hybrid type, releasing the glycan and leaving one GlcNAc attached to the protein. The activity of EF2863 is similar to that of the well known deglycosylating enzyme EndoH from Streptomyces plicatus. According to the CAZy nomenclature, the enzyme is designated EfEndo18A.

机译：先前已证明粪肠球菌产生分泌的糖苷内切酶，使细菌能够从糖蛋白中去除N-连接的聚糖。可能负责这种活性的一种酶是EF0114，其包含典型的GH18内切糖苷酶结构域和GH20结构域。我们已经分析了另一种候选药物EF2863，并表明该预测的单结构域GH18蛋白是内切β-N-乙酰氨基葡糖苷酶。 EF2863水解高甘露糖和杂合型N联聚糖中两个N-乙酰氨基葡糖胺（GlcNAc）之间的糖苷键，释放出聚糖，并使一个GlcNAc附着在蛋白质上。 EF2863的活性类似于褶皱链霉菌的众所周知的去糖基化酶EndoH的活性。根据CAZy命名法，该酶称为EfEndo18A。

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《FEMS Microbiology Letters》 |2011年第2期|共7页
作者
B?hle L.A.; Mathiesen G.; Vaaje-Kolstad G.; Eijsink V.G.;
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正文语种 eng
中图分类微生物学;
关键词
Endo-β-N-acetylglucosaminidase; Enterococcus faecalis V583; Glycoprotein; N-linked glycan;

机译：内-β-N-乙酰氨基葡糖苷酶;粪肠球菌V583;糖蛋白;N-连接聚糖;

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1. An endo-β-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans [J] . B?hle L.A., Mathiesen G., Vaaje-Kolstad G., FEMS Microbiology Letters . 2011,第2期

机译：粪肠球菌V583的内切β-N-乙酰氨基葡萄糖苷酶，负责水解高甘露糖和杂合型N-连接聚糖
2. Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N'-Acetylchitobiosyl Unit [J] . Yoshinobu Kimura, Yuki Takeoka, Masami Inoue Bioscience, Biotechnology, and Biochemistry . 2011,第5期

机译：拟南芥中推定的内切-β-N-乙酰氨基葡糖苷酶（ENGase）基因的双重敲除：ENGase活性的丧失诱导了高甘露糖型游离N-聚糖的积累，带有N，N'-乙酰壳多糖的单元。
3. Molecular Cloning and Gene Expression Analysis of Tomato Endo-β-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening [J] . Kosuke Nakamura, Masami Inoue, Megumi Maeda Bioscience, Biotechnology, and Biochemistry . 2009,第2期

机译：番茄内切-β-N-乙酰氨基葡糖苷酶（一种内切糖苷酶，参与高果糖型游离N-聚糖的生产）的分子克隆和基因表达分析
4. Production of an Endo-β-N-Acetylglucosaminidase Activity Mediates Growth of Enterococcus faecalis on a High-Mannose-Type Glycoprotein [O] . Gretta Roberts, Edward Tarelli, Karen A. Homer, 2000

机译：内源性β-N-乙酰氨基葡糖苷酶活性的产生介导粪肠球菌在高甘露糖型糖蛋白上的生长
5. Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes inArabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type FreeN-Glycans BearingN,N′-Acetylchitobiosyl Unit [O] . Yoshinobu KIMURA, Yuki TAKEOKA, Masami INOUE, 2011

机译：推定的endo-β-n-乙酰葡糖胺酶（engase）基因的双敲除inarabidopsis inalabidopsis：engase活性损失诱导的高甘露糖型含有n的含量，N'-乙酰基核糖氧基单位

An endo-β-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans

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