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首页> 外文期刊>FEMS Microbiology Ecology >Methane-cycling communities in a permafrost-affected soil on Herschel Island, Western Canadian Arctic: Active layer profiling of mcrA and pmoA genes
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Methane-cycling communities in a permafrost-affected soil on Herschel Island, Western Canadian Arctic: Active layer profiling of mcrA and pmoA genes

机译：加拿大西部北极地区Herschel岛永久冻土土壤中的甲烷循环群落：mcrA和pmoA基因的活性层分析

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In Arctic wet tundra, microbial controls on organic matter decomposition are likely to be altered as a result of climatic disruption. Here, we present a study on the activity, diversity and vertical distribution of methane-cycling microbial communities in the active layer of wet polygonal tundra on Herschel Island. We recorded potential methane production rates from 5 to 40 nmol h ~(-1) g ~(-1) wet soil at 10 °C and significantly higher methane oxidation rates reaching values of 6-10 μmol h ~(-1) g ~(-1) wet soil. Terminal restriction fragment length polymorphism (T-RFLP) and cloning analyses of mcrA and pmoA genes demonstrated that both communities were stratified along the active layer vertical profile. Similar to other wet Arctic tundra, the methanogenic community hosted hydrogenotrophic (Methanobacterium) as well as acetoclastic (Methanosarcina and Methanosaeta) members. A pronounced shift toward a dominance of acetoclastic methanogens was observed in deeper soil layers. In contrast to related circum-Arctic studies, the methane-oxidizing (methanotrophic) community on Herschel Island was dominated by members of the type II group (Methylocystis, Methylosinus, and a cluster related to Methylocapsa). The present study represents the first on methane-cycling communities in the Canadian Western Arctic, thus advancing our understanding of these communities in a changing Arctic.
机译：在北极湿地苔原中，由于气候破坏，有机物分解的微生物控制可能会改变。在这里，我们对赫歇尔岛上湿多边形苔原活动层中甲烷循环微生物群落的活性，多样性和垂直分布进行研究。我们记录了在10°C下从5到40 nmol h〜（-1）g〜（-1）湿土壤的潜在甲烷生成速率，甲烷氧化速率显着提高，达到6-10μmolh〜（-1）g〜 （-1）潮湿的土壤。末端限制性片段长度多态性（T-RFLP）和mcrA和pmoA基因的克隆分析表明，这两个社区都沿活性层垂直剖面分层。类似于其他湿北极苔原，产甲烷群落也有氢营养型（Methanobacterium）和碎裂质（Methanosarcina和Methanosaeta）成员。在较深的土壤层中观察到明显向着乙酰碎屑产甲烷菌的转变。与相关的北极圈外研究相反，赫歇尔岛上的甲烷氧化（甲烷营养）群落主要由II型组成员（甲基囊藻，甲基ososinus和与甲基capsa相关的簇）主导。本研究是加拿大西部北极地区首个有关甲烷循环社区的研究，从而加深了我们对变化中的北极这些社区的了解。

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