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首页> 外文期刊>FEMS Microbiology Ecology >An assessment of RNA content in Prymnesium parvum, Prymnesium polylepis, cf. Chattonella sp. and Karlodinium veneficum under varying environmental conditions for calibrating an RNA microarray for species detection
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An assessment of RNA content in Prymnesium parvum, Prymnesium polylepis, cf. Chattonella sp. and Karlodinium veneficum under varying environmental conditions for calibrating an RNA microarray for species detection

机译：小球藻，小球藻中RNA含量的评估Chattonella sp。和在不同环境条件下的头孢霉菌用于校准用于物种检测的RNA微阵列

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摘要

Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P. polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and inorganic nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.
机译：当识别浮游植物需要电子显微镜检查以验证特定形态特征时，传统的识别和枚举方法可能有些模棱两可。浮游植物属（七叶植物科）的成员，水藻科的成员和裸鞭毛鞭毛藻类是这类浮游植物的例子，它们的识别可能很困难。传统的基于显微镜的识别方法的一种替代方法是使用分子方案来检测目标物种。测量细胞DNA和RNA含量的方法可用于估计样品中存在的细胞数量。这项研究调查了小白屈菜（P. polylepis）中RNA产量的变化。 Chattonella sp。和在不同的光照，温度，盐度和无机营养条件下生长的香叶Karlodinium v​​eneficum细胞。该信息用于校准微阵列上用于检测有毒藻类（MIDTAL）的各种寡核苷酸探针的信号强度，该寡核苷酸正在开发中，以协助国家监测机构并提供更快的方法来识别和量化有害浮游植物在水柱样品中。

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