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首页> 外文期刊>FEMS Microbiology Letters >Ploidy and chromosomal number in Tuber aestivum
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Ploidy and chromosomal number in Tuber aestivum

机译：马铃薯块茎的倍性和染色体数目

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摘要

Chromosome number is generally considered to be the most accurate and direct measure of ploidy. In fungi, however, counting of chromosomes is difficult and inaccurate because of their small size. To date truffles have been characterized using molecular approaches (analysis of DNA and PCR-based techniques) in order to find out differences among species, but not by a cytogenetical approach. Although the small size of truffle chromosomes is of some hindrance for a cytogenetic study, in the present work Tuber aestivum chromosome counts were determined in metaphase configurations from haploid nuclei of ascospores by aceto-orcein staining. Nuclear chromosome number and topography were also evaluated by propidium iodide DNA staining using confocal microscopy. Tuber aestivum was found to possess a basic number of 5 or 6 chromosomes, medium length less than or equal to 0.95 mu m. The karyology of ascospores during their developmental stages was also investigated. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. [References: 16]
机译：通常认为染色体数是最准确，最直接的倍性度量。但是，在真菌中，由于染色体尺寸小，因此难以计数且不准确。迄今为止，为了发现菌种之间的差异，已经使用分子方法（基于DNA和基于PCR的技术分析）对松露进行了表征，但并未通过细胞遗传学方法对其进行表征。尽管块菌染色体的小尺寸对于细胞遗传学研究有一定的障碍，但在目前的工作中，通过乙酰-大黄素染色，从子囊的单倍体核的中期构型中确定了块茎小麦的染色体计数。还使用共聚焦显微镜通过碘化丙啶DNA染色评估了核染色体数和形貌。发现马铃薯块茎具有5或6条染色体的基本数目，中等长度小于或等于0.95μm。还研究了子囊孢子在其发育阶段的核力学。 （C）1998年欧洲微生物学会联合会。由Elsevier Science B.V.保留所有权利。 [参考：16]

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