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首页> 外文期刊>Biochemistry >A DESIGNED HETEROTRIMERIC COILED COIL
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A DESIGNED HETEROTRIMERIC COILED COIL

机译：设计的异三元卷材

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摘要

Principles that guide folding of coiled coils were tested by designing three peptides that preferentially associate with each other to form a heterotrimeric coiled coil. The core positions of the designed helices contained residues that promote formation of trimeric coiled coils. Ionic interactions were employed to mediate heterospecificity, and negative design was used to favor formation of the heterotrimer over alternative arrangements. A program was written to select sequences that maximized the number of attractive interhelical interactions in a parallel heterotrimer and the number of repulsive electrostatic interactions in alternative species. Solution studies indicate that an equimolar mixture of the three peptides forms a helical trimer with high specificity and stability. These results validate the principles used to guide the design and suggest that the heterotrimer may serve as a useful, autonomous trimerization domain.
机译：通过设计优先彼此结合形成异三聚体卷曲螺旋的三种肽，测试了引导卷曲螺旋折叠的原理。设计的螺旋的核心位置包含促进三聚体卷曲螺旋形成的残基。离子相互作用被用来介导异源特异性，并且负设计被用于在替代方案上有利于异源三聚体的形成。编写了一个程序来选择序列，以最大化平行异三聚体中有吸引力的螺旋间相互作用的数量以及替代物种中排斥性静电相互作用的数量。溶液研究表明，三种肽的等摩尔混合物形成具有高特异性和稳定性的螺旋三聚体。这些结果验证了用于指导设计的原理，并表明异三聚体可以用作有用的自主三聚域。

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