...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEMS Yeast Research >Stimulation of astaxanthin formation in the yeast Xanthophyllomyces dendrorhous by the fungus Epicoccum nigrum
【24h】

Stimulation of astaxanthin formation in the yeast Xanthophyllomyces dendrorhous by the fungus Epicoccum nigrum

机译：黑木耳真菌刺激树突状黄单胞菌中虾青素的形成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A fungal contaminant on an agar plate containing colonies of Xanthophyllomyces dendrorhous markedly increased carotenoid production by yeast colonies near to the fungal growth. Spent-culture filtrate from growth of the fungus in yeast-malt medium also stimulated carotenoid production by X. dendrorhous. Four X. dendrorhous strains including the wild-type UCD 67-385 (ATCC 24230), AF-1 (albino mutant, ATCC 96816), Yan-1 (beta-carotene mutant, ATCC 96815) and CAX (astaxanthin overproducer mutant) exposed to fungal concentrate extract enhanced astaxanthin up to approximately 40% per unit dry cell weight in the wild-type strain and in CAX Interestingly, the fungal extract restored astaxanthin biosynthesis in non-astaxanthin-producing mutants previously isolated in our laboratory, including the albino and the beta-carotene mutant. The fungus was identified as Epicoccum nigrum by morphology of sporulating cultures, and the identity confirmed by genetic characterization including rDNA sequencing analysis of the large-subunit (LSU), the internal transcribed spacer, and the D1/D2 region of the LSU. These E. nigrum rDNA sequences were deposited in GenBank under accesssion numbers AF338443, AY093413 and AY093414. Systematic rDNA homology alignments were performed to identify fungi related to E. nigrum. Stimulation of carotenogenesis by E. nigrum and potentially other fungi could provide a novel method to enhance astaxanthin formation in industrial fermentations of X. dendrorhous and Phaffia rhodozyma
机译：琼脂平板上的一种真菌污染物含有黄腐菌的集落，通过靠近真菌生长的酵母菌落显着增加了类胡萝卜素的产生。酵母在麦芽培养基中真菌生长所产生的废培养滤液也刺激了X. dendrorhous产生类胡萝卜素。暴露的四种X.dendrorhous菌株包括野生型UCD 67-385（ATCC 24230），AF-1（白化突变体，ATCC 96816），Yan-1（β-胡萝卜素突变体，ATCC 96815）和CAX（黄嘌呤高产突变体）真菌浓缩物提取物在野生型菌株和CAX中将虾青素增强至每单位干细胞重量约40％，有趣的是，真菌提取物恢复了以前在我们实验室中分离出的非产黄嘌呤的突变体中虾青素的生物合成，包括白化病和β-胡萝卜素突变体。通过孢子培养的形态将真菌鉴定为黑葡萄球菌，并通过遗传表征（包括大亚基（LSU）的rDNA测序分析，内部转录间隔区和LSU的D1 / D2区）确认了身份。这些黑曲霉rDNA序列以登录号AF338443，AY093413和AY093414保藏在GenBank中。进行系统的rDNA同源性比对，以鉴定与黑质大肠杆菌相关的真菌。黑曲霉和潜在的其他真菌对类胡萝卜素的刺激可以提供一种新的方法来增强树突状支原体和红发夫酵母的工业发酵中虾青素的形成

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号