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首页> 外文期刊>Calcified tissue international. >Expression of mRNAs for the alpha 1 subunit of voltage-gated calcium channels in human osteoblast-like cell lines and in normal human osteoblasts.
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Expression of mRNAs for the alpha 1 subunit of voltage-gated calcium channels in human osteoblast-like cell lines and in normal human osteoblasts.

机译：电压门控钙通道的α1亚基的mRNA在人成骨细胞样细胞系和正常人成骨细胞中的表达。

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The activation of osteoblast calcium channels by many bone regulatory factors suggests an important role for intracellular calcium signaling in the control of bone remodeling. At least six different genes for the alpha 1 subunit of voltage-gated calcium channels have been cloned including L-type (alpha 1S, alpha 1C, and alpha 1D) and non-L-type (alpha 1A, alpha 1B, and alpha 1E) isoforms. The goal of the present study was to identify which of these calcium channel isoforms are transcribed in human osteoblast-like cell lines (hFOB, MG-63, SAOS-2, TE-85, G-292) and in cultures of normal human osteoblasts. Reverse transcriptase-PCR was used to amplify sequences corresponding to each of the alpha 1 subunits using isoform specific primers. The products of the PCR reaction were cloned and sequenced to verify their identify and used to probe southern blots of the PCR reactions. The results indicate that among the different types of osteoblast-like cells examined, two calcium channel isoforms were always expressed (alpha 1C and alpha 1A), three isoforms were variably expressed (alpha 1S, alpha 1D and alpha 1B), and one isoform was not expressed in any of the osteoblast-like cells (alpha 1E) but was easily detected in human brain controls. Our results indicate that mRNAs for multiple calcium channel alpha 1 subunits are expressed in human osteoblasts, including both L-type and non-L-type isoforms. In addition, significant heterogeneity exists between the different osteoblast cell models examined in the type and mRNA abundance of the different calcium channel isoforms.
机译：许多骨调节因子对成骨细胞钙通道的激活提示细胞内钙信号传导在骨重塑控制中的重要作用。已克隆了电压门控钙通道的alpha 1亚基的至少六个不同基因，包括L型（alpha 1S，alpha 1C和alpha 1D）和非L型（alpha 1A，alpha 1B和alpha 1E） ）异构体。本研究的目的是鉴定在人成骨样细胞系（hFOB，MG-63，SAOS-2，TE-85，G-292）和正常人成骨细胞培养物中转录了哪些钙通道亚型。逆转录酶PCR用于使用同工型特异性引物扩增对应于每个α1亚基的序列。将PCR反应的产物克隆并测序以验证其身份，并用于探测PCR反应的Southern印迹。结果表明，在所检查的不同类型的成骨样细胞中，总是表达两种钙通道亚型（α1C和α1A），三种亚型可变表达（α1S，α1D和α1B），一种亚型是在任何成骨细胞样细胞（alpha 1E）中均未表达，但在人脑对照中很容易检测到。我们的结果表明，多个钙通道α1亚基的mRNA在人类成骨细胞中表达，包括L型和非L型亚型。此外，在不同钙通道亚型的类型和mRNA丰度方面，不同成骨细胞模型之间存在显着的异质性。

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