...
首页> 外文期刊>Biochemistry >DNA bending by GCN4 mutants bearing cationic residues
【24h】

DNA bending by GCN4 mutants bearing cationic residues

机译:GCN4突变体携带阳离子残基的DNA弯曲

获取原文
获取原文并翻译 | 示例
           

摘要

Transcription activation is thought to require DNA bending to promote the interaction of upstream activators and the basal transcription machinery. Previous experiments have shown that some members of the bZIP family of DNA binding proteins bend DNA, while others do not. We are exploring the possibility that electrostatic effects play a role in these differences. The yeast bZIP transcription factor GCN4 does not induce DNA bending in vitro. Substitution of basic residues for three neutral amino acids of GCN4 confers the ability to bend DNA. This result is consistent with a model of induced DNA bending wherein excess positive charge in proximity to one face of the double helix neutralizes local phosphate diester anions resulting in a laterally asymmetric charge distribution along the DNA. Previous data suggest that such an unbalanced charge distribution results in collapse of the DNA toward the neutralized surface. Interpretations of the present data are discussed. Our result supports the hypothesis that electrostatic interactions can play a key role in DNA bending by bZIP proteins.
机译:转录激活被认为需要弯曲DNA以促进上游激活子和基础转录机制的相互作用。先前的实验表明,bZIP DNA结合蛋白家族的某些成员使DNA弯曲,而另一些则没有。我们正在探索静电效应在这些差异中起作用的可能性。酵母bZIP转录因子GCN4在体外不诱导DNA弯曲。 GCN4的三个中性氨基酸的碱性残基替代赋予弯曲DNA的能力。该结果与诱导的DNA弯曲的模型一致,在该模型中,双螺旋的一个面附近的过量正电荷中和了局部磷酸二酯阴离子,导致沿DNA横向不对称分布。先前的数据表明,这种不平衡的电荷分布会导致DNA向中和的表面塌陷。讨论了本数据的解释。我们的结果支持以下假设:静电相互作用可以在bZIP蛋白弯曲DNA中发挥关键作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号