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首页> 外文期刊>Biochemistry >Spin-label studies on the anchoring and lipid-protein interactions of avidin with N-biotinylphosphatidylethanolamines in lipid bilayer membranes
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Spin-label studies on the anchoring and lipid-protein interactions of avidin with N-biotinylphosphatidylethanolamines in lipid bilayer membranes

机译：亲和素与脂质双层膜中N-生物素磷脂酰乙醇胺的锚定和脂蛋白相互作用的自旋标记研究

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摘要

The specific binding of hen egg white avidin to phosphatidylcholine lipid membranes containing spin-labeled N-biotinylphosphatidylethanolamines (biotin-PESLs) was investigated by using ESR spectroscopy. Spin-labeled biotin-PEs were prepared with the nitroxide group at position C-5, C-8, C-10, C-12, or C-14 of the sn-2 chain and were incorporated at 1 mol % in lipid bilayer membranes of dimyristoylphosphatidylcholine. Binding of avidin produced a strong and selective restriction of the biotin-PE lipid mobility at all positions of chain labeling, as shown by the ESR spectra recorded in the fluid lipid phase. The spectral components of the fraction of the biotin-PESLs that were not complexed by avidin indicated that the mobility of the bulk membrane lipids was unperturbed by binding avidin, as demonstrated by difference spectroscopy. Comparison of the positional profiles and temperature dependences of the outer hyperfine splittings from the biotin-PESLs suggests that the C-12 and C-14 positions of the avidin-bound biotin-PEs are in register with the C-5 and C-7/C-6 positions, respectively, of the chains of the bulk membrane lipids. This indicates that the biotin-PEs are partially withdrawn from the membrane, with a vertical displacement of ca. 7-8 A, on complexation with avidin. In addition, the specific lipid-protein interaction with avidin results in a selective reduction in the rates of lipid chain motion, as shown by the increased ESR line widths. These data define the way in which avidin is anchored to lipid membranes containing biotin-PEs.
机译：通过使用ESR光谱研究了鸡蛋清抗生物素蛋白与含有自旋标记N-生物素磷脂酰乙醇胺（生物素-PESLs）的磷脂酰胆碱脂质膜的特异性结合。制备自旋标记的生物素-PEs，其硝化基团位于sn-2链的C-5，C-8，C-10，C-12或C-14位，并以1 mol％的比例掺入脂质双层二豆基磷脂酰胆碱的膜。如在流体脂质相中记录的ESR谱所示，抗生物素蛋白的结合在链标记的所有位置上都强烈且选择性地限制了生物素-PE脂质的迁移。未由抗生物素蛋白复合的部分生物素-PESLs的光谱成分表明，结合的抗生物素蛋白不会干扰大分子膜脂质的迁移，如差示光谱法所示。生物素-PESL的外部超细碎片的位置分布和温度依赖性的比较表明，抗生物素蛋白结合的生物素-PE的C-12和C-14位置与C-5和C-7 /整体膜脂质的链的C-6位置。这表明生物素-PEs从膜中部分撤出，垂直位移约为。 7-8A，与抗生物素蛋白复合。另外，与抗生物素蛋白的特异性脂蛋白相互作用导致脂链运动速率的选择性降低，如增加的ESR线宽所示。这些数据定义了抗生物素蛋白锚定到含有生物素-PE的脂质膜上的方式。

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