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首页> 外文期刊>Calcified tissue international. >New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy
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New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy

机译：共焦激光扫描显微镜和微分干涉对比显微镜联合评估的新骨形成和显微组织

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摘要

Bone is a mineralized connective tissue that is continuously and microstructurally remodeled. Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone patho-genesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann's channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies.
机译：骨是矿化的结缔组织，可以连续地进行微观结构重塑。在诸如骨质疏松症，骨坏死，骨折修复和骨Paget病的病理性骨病中会发生骨形成和微结构的改变。对骨形成和微结构的正确而客观的评估将提供对了解骨病理发生和重塑的认识。在这里，使用共聚焦激光扫描显微镜（CLSM）在体内多个连续的多色标记的样品中评估了离体的新骨形成及其微结构，与常规荧光显微镜（CFM）相比，该显微镜生成的厚骨切片图像更清晰，更可靠。有趣的是，使用CLSM阐明了骨微结构特征的精细细节，包括矿化前沿，静态与活动骨以及Volkmann通道，该方法定义了形态变化与功能之间的关系，并与差分干涉对比显微镜相结合。此外，CLSM提供了骨形成的客观评估，例如与CFM相比，兔模型中新骨形成的标记区域的比率。总之，结合使用CLSM和DIC显微镜检查，可以更充分地评估新骨的形成及其微观结构。

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