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首页> 外文期刊>Canadian journal of microbiology >Reduction of linoleic acid inhibition in production of conjugated linoleicacid by Propionibacterium freudenreichii ssp shermanii
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Reduction of linoleic acid inhibition in production of conjugated linoleicacid by Propionibacterium freudenreichii ssp shermanii

机译:freudenreichii ssp shermanii减少对共轭亚油酸生产中亚油酸的抑制

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摘要

A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 mug.mL(-1) LA, which was converted to CLA with 57%-87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%-90% of the CLA produced. The feasibility of the method was demonstrated also in de Man - Rogosa-Sharpe (MRS) broth.
机译:一种利用弗氏丙酸杆菌的生长培养物从亚油酸(LA)生产共轭亚油酸(CLA)的方法。开发了shermanii JS。通过将LA分散在足够浓度的聚氧乙烯脱水山梨糖醇单油酸酯清净剂中,可以消除LA的生长抑制作用。对于所用的乳清渗透介质,最佳的LA:洗涤剂比为1:15(w / w)。结果,该培养物耐受至少1000 mug.mL(-1)LA,将其转化为CLA的效率为57%-87%。顺式9,反式11和反式9,顺式11异构体占所产生CLA的85%-90%。该方法的可行性也在de Man-Rogosa-Sharpe(MRS)肉汤中得到了证明。

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