Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: Monitoring the optical properties of the complex from bacterial cell to crystal

McAuley-Hecht KE.; Ridge JP.; Prince SM.; Hunter CN.; Isaacs NW. Cogdell RJ.; Jones MR.; Fyfe PK.

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Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: Monitoring the optical properties of the complex from bacterial cell to crystal

机译：球形缺乏球形红细菌细菌菌株的野生型和突变反应中心的结构研究：监测从细菌细胞到晶体的复合物的光学性质

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Reaction centers have been crystallized from the antenna-deficient RCO2 strain of Rhodobacter sphaeroides, and a structural model has been constructed at 2.6 Angstrom resolution. The antenna-deficient strain allows assessment of the structural integrity of the reaction center at each stage in the purification-crystallization procedure. Spectroscopic evidence indicates that the properties of the reaction center bacteriopheophytins and the primary donor bacteriochlorophylls are modified somewhat on removal of the protein complex from the membrane and that these changes are carried through to the crystal form of the reaction center. The structure of a FM197R/YM177F mutant reaction center has also been determined to 2.55 Angstrom resolution. The mutant complex shows an unexpected change in structure, with a significant reorientation of the new arginine, the incorporation of a new water molecule into the structure, and rotation of the 2-acetyl carbonyl group of one of the primary donor bacteriochlorophylls to a more out-of-plane geometry. Changes in the optical spectrum of the FM197R/YMl77F reaction center are discussed with respect to the altered structure of the complex. [References: 59]

机译：反应中心已从球形球形红球菌的缺乏天线的RCO2菌株中结晶出来，并以2.6埃的分辨率构建了结构模型。缺乏天线的应变可以评估纯化结晶过程中每个阶段反应中心的结构完整性。光谱证据表明，当从膜上除去蛋白质复合物时，反应中心细菌脱镁叶绿素和主要供体细菌叶绿素的性质有所改变，并且这些变化一直传递到反应中心的晶体形式。 FM197R / YM177F突变反应中心的结构也已确定为2.55埃分辨率。突变体复合物显示出意想不到的结构变化，其中新精氨酸发生了明显的重新定向，新的水分子掺入了结构中，主要供体细菌叶绿素之一的2-乙酰基羰基向更多方向旋转。平面几何。关于配合物结构的变化，讨论了FM197R / YM177F反应中心光谱的变化。 [参考：59]

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《Biochemistry》 |1998年第14期|共11页
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McAuley-Hecht KE.; Ridge JP.; Prince SM.; Hunter CN.; Isaacs NW. Cogdell RJ.; Jones MR.; Fyfe PK.;
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1. Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: Monitoring the optical properties of the complex from bacterial cell to crystal [J] . McAuley-Hecht KE., Ridge JP., Prince SM., Biochemistry . 1998,第14期

机译：球形缺乏球形红细菌细菌菌株的野生型和突变反应中心的结构研究：监测从细菌细胞到晶体的复合物的光学性质
2. Electronic and Vibronic Coupling of the Special Pair of Bacteriochlorophylls in Photosynthetic Reaction Centers from Wild-Type and Mutant Strains of Rhodobacter Sphaeroides [J] . E. T. Johnson, F. Muh, E. Nabedryk, The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2002,第45期

机译：球形红球菌野生型和突变株光合反应中心中特定对细菌叶绿素的电子和振动耦合
3. Low-Temperature Pulsed EPR Study at 34 GHz of the Triplet States of the Primary Electron Donor P_(865) and the Carotenoid in Native and Mutant Bacterial Reaction Centers of Rhodobacter sphaeroides [J] . Aliaksandr Marchanka, Mark Paddock, Wolfgang Lubitz Biochemistry . 2007,第51期

机译：球形脉冲红细菌天然和突变细菌反应中心中初级电子供体P_（865）和类胡萝卜素三态态在34 GHz的低温脉冲EPR研究
4. Spectral Properties of the Rhodobacter Sphaeroides Mutant Photo-Reaction Center with Double Amino Acid Substitution I(L177)H+H(L173)L [C] . Tatiana Y Fufina, Lyudmila G Vasilieva, Ravil A Khatypov, Photosynthesis research for food, fuel and future . 2010

机译：具有双氨基酸取代I（L177）H + H（L173）L的球形球形红细菌突变体光反应中心的光谱性质
5. Characterization of Multi-nuclear Manganese-binding Bacterial Reaction Centers from Rhodobacter sphaeroides [D] . Espiritu, Eduardo 2019

机译：球形球形红细菌对多核锰结合细菌反应中心的表征
6. Pathway of proton transfer in bacterial reaction centers: second-site mutation Asn-M44-->Asp restores electron and proton transfer in reaction centers from the photosynthetically deficient Asp-L213-->Asn mutant of Rhodobacter sphaeroides. [O] . S H Rongey, M L Paddock, G Feher, 2018

机译：细菌反应中心质子转移的途径：二级位点突变Asn-M44-> Asp恢复了光合缺陷型球形红球菌Asp-L213-> Asn突变体在反应中心的电子和质子转移。
7. Spectroscopic characterization of reaction center crystals from the carotenoid-containing wild-type strain Rhodobacter sphaeroides Y [O] . Reiss-Husson F., Mäntele W. 1988

机译：含类胡萝卜素的野生型球形红球菌Y的反应中心晶体的光谱表征
8. Crystallographic Studies of the Photosynthetic Reaction Center from R. Sphaeroides [R] . Chang, C. H. , Tiede, D. , Tang, J. , 1986

机译：R. sphaeroides光合反应中心的晶体学研究

Structural studies of wild-type and mutant reaction centers from an antenna-deficient strain of Rhodobacter sphaeroides: Monitoring the optical properties of the complex from bacterial cell to crystal

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