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首页> 外文期刊>Fitoterapia >Determination of dehydroevodiamine in Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography and classification of the samples by using hierarchical clustering analysis
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Determination of dehydroevodiamine in Evodia rutaecarpa (Juss.) Benth by high performance liquid chromatography and classification of the samples by using hierarchical clustering analysis

机译：高效液相色谱法测定吴茱Ben药材中的脱氢戊二胺，采用层次聚类分析对样品进行分类

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摘要

A simple, sensitive and accurate liquid chromatographic method with photodiode-array detection was developed for determination of dehydroevodiamine with detection wavelength at 368 nm and column temperature at 30 degrees C. The separation was carried out on an Agilent Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm) together with a C(18) guard column. The mobile phase was acetonitrile-water (containing 30 mM sodium acetate trihydrate and 0.15% acetic acid) in the ratio of 30:70 (v/v) delivered at a flow rate of 1 mL/min. Excellent linear behavior was observed over the concentration range investigated, with correlation coefficient (R(2))=0.9998. This validated method was applied to determine the contents of dehydroevodiamine in 36 samples from different regions of China, and hierarchical clustering analysis was firstly used to classify and differentiate Evodia rutaecarpa samples. The analysis is specific and can be successfully applied to analyze E. rutaecarpa which is helpful for quality control of the herb.
机译：开发了一种简单，灵敏，准确的液相色谱-光电二极管阵列检测方法，用于测定脱氢戊二胺，检测波长为368 nm，柱温为30摄氏度。分离是在Agilent Zorbax SB-C（18）色谱柱上进行的（250 mm x 4.6 mm，5微米）和C（18）保护柱。流动相是乙腈-水（含有30 mM乙酸钠三水合物和0.15％乙酸），以30:70（v / v）的比例以1 mL / min的流速输送。在研究的浓度范围内观察到优异的线性行为，相关系数（R（2））= 0.9998。该验证方法用于测定来自中国不同地区的36个样品中的脱氢戊二胺含量，并且首先使用层次聚类分析对吴茱E样品进行分类和区分。该分析是特定的，可以成功地用于分析芸苔（E. rutaecarpa），这有助于草药的质量控制。

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