...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Formation of CLC-0 chloride channels from separated transmembrane and cytoplasmic domains
【24h】

Formation of CLC-0 chloride channels from separated transmembrane and cytoplasmic domains

机译：由分离的跨膜和胞质域形成CLC-0氯通道

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CLC-0, a member of the CLC family of Cl--conducting ion channels, consists of an N-terminal hydrophobic core and a C-terminal region that is thought to be cytoplasmic. This study provides evidence that the C-terminal region is a mechanistically relevant cytoplasmic domain of the CLC-0 ion channel. Both a point mutation and a 37-residue deletion in this region cause drastic alterations in voltage-dependent gating of CLC-0 current expressed in Xenopus oocytes. CLC-0 current is not observed when the entire C-terminal region is deleted, but functional channels are efficiently reconstituted by co-injection of separate cRNA constructs encoding the N-terminal transmembrane and the C-terminal cytoplasmic domains. Moreover, reconstitution of CLC-0 can be achieved by co-injection of cRNA encoding the transmembrane domain along with Escherichia coli-expressed C-terminal domain polypeptide. [References: 52]
机译：CLC-0是CLC导电离子通道家族的成员，由N端疏水核和C端区域组成，被认为是细胞质的。这项研究提供的证据表明，C末端区域是CLC-0离子通道的机械相关胞质域。在该区域中的点突变和37个残基的缺失均引起爪蟾卵母细胞中表达的CLC-0电流的电压依赖性门控的急剧变化。当删除整个C端区域时，未观察到CLC-0电流，但是通过共同注射编码N端跨膜和C端胞质域的单独cRNA构建体，有效重建了功能通道。而且，可以通过共同注射编码跨膜结构域的cRNA和表达大肠杆菌的C末端结构域多肽来实现CLC-0的重建。 [参考：52]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号