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首页> 外文期刊>Fluid Phase Equilibria >Partitioning of lactate dehydrogenase from bovine heart crude extract by polyethylene glycol-citrate aqueous two-phase systems
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Partitioning of lactate dehydrogenase from bovine heart crude extract by polyethylene glycol-citrate aqueous two-phase systems

机译：聚乙二醇柠檬酸酯水相两相法分离牛心粗提物中的乳酸脱氢酶

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摘要

Aqueous two-phase systems (ATPS) composed of polyethylene glycol (PEG)-citrate have been used for enzyme partitioning studies. The behavior of lactate dehydrogenase (LDH) from bovine heart crude extract was analyzed using a two-level factorial design in which the PEG molar mass and concentration, the citrate concentration were selected as independent variables, while the purification factor, the partition coefficient (K) and the activity yield were selected as responses. The statistical analysis revealed the effect of PEG molar mass on K. LDH exhibited a better partitioning toward PEG-rich phase and the highest K value (1079.81) was obtained with 42% (w/w) PEG 400 and 7.5% (w/w) citrate concentration. PEG molar mass also influenced the purification factor of the enzyme in the top phase. Possibly these ATPS remove inhibitors present in the extract affording higher enzyme yield.
机译：由柠檬酸聚乙二醇（PEG）组成的水两相系统（ATPS）已用于酶分配研究。采用二级因子设计分析牛心粗提物中乳酸脱氢酶（LDH）的行为，其中选择PEG摩尔质量和浓度，柠檬酸盐浓度作为自变量，而纯化因子，分配系数（K ），然后选择活性产量作为响应。统计分析显示了PEG摩尔质量对K的影响。LDH表现出更好的向富PEG相的分配，并且以42％（w / w）PEG 400和7.5％（w / w）可获得最高的K值（1079.81）。 ）柠檬酸盐浓度。 PEG摩尔质量也影响了顶相中酶的纯化因子。可能这些ATPS去除了提取物中存在的抑制剂，从而提供了更高的酶产率。

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