...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Folia Biologica: A Quarterly Journal of Biological Research >DNA Methylation, Histone Modifications and Behaviour of AKAP95 during Mouse Oocyte Growth and Upon Nuclear Transfer of Foreign Chromatin into Fully Grown Prophase Oocytes*
【24h】

DNA Methylation, Histone Modifications and Behaviour of AKAP95 during Mouse Oocyte Growth and Upon Nuclear Transfer of Foreign Chromatin into Fully Grown Prophase Oocytes*

机译：小鼠卵母细胞生长过程中以及外源染色质核转移到完全生长的前卵母细胞中时，DNA甲基化，组蛋白修饰和AKAP95的行为*

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The poor efficiency of mammalian cloning is due to inappropriate/incomplete epigenetic reprogramming of the donor chromatin. As the success in reprogramming of the donor nucleus may require activity of similar mechanisms which reprogram the chromatin in the course of gametogenesis, we decided to follow the status of some epigenetic markers in the late phase of oogenesis in mice, i.e. in prophase oocytes during their growth and after completion of the growth phase. Our analysis reveals an increase in the level of global DNA methylation starting in oocytes with diameters around 60μm which was further elevated until completion of oocyte growth. A similar increase was observed in respect to the acetylation of histone H4. On the other hand, the methylation of histone H4 Arg3 was constantly high until the end of oocyte growth, although it differed between fully grown oocytes depending on the type of spatial chromatin organization. We have also studied the AKAP95 protein which was abundant at earlier stages but decreased in fully grown oocytes according to changes in their chromatin organization. The nuclear transfer of different types of donor nuclei with hypomethylated DNA into fully grown prophase oocytes did not increase the global level of methylation of transferred foreign chromatin, regardless if the recipient oocyte was devoid of its own nucleus or its nucleus was left intact. This suggests a major problem in the ability of recipient oocytes to modify donor DNA methylation.
机译：哺乳动物克隆效率低下是由于供体染色质的表观遗传重编程不适当/不完整。由于成功地重编程供体核可能需要在配子发生过程中重新激活染色质的类似机制的活性，因此我们决定在小鼠卵子发生的后期，即在卵母细胞的前期卵母细胞中追踪某些表观遗传标记的状态。成长阶段以及成长阶段完成之后。我们的分析显示，直径在60μm左右的卵母细胞中总体DNA甲基化水平增加，并进一步升高直至卵母细胞生长完成。关于组蛋白H4的乙酰化，观察到类似的增加。另一方面，组蛋白H4 Arg3的甲基化一直持续到卵母细胞生长结束，尽管完全成熟的卵母细胞根据空间染色质组织的类型而有所不同。我们还研究了AKAP95蛋白，该蛋白在早期阶段丰富，但在完全生长的卵母细胞中会根据其染色质组织的变化而减少。不论受体卵母细胞没有自己的核还是保留完整的核，无论是带有低甲基化DNA的不同类型供体核向完全生长的前卵母细胞的核转移，都不会增加已转移外源染色质的总体甲基化水平。这表明受体卵母细胞修饰供体DNA甲基化能力的主要问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号