...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research >Studies on the toxicity of deoxynivalenol (DON), sodium metabisulfite, DON-sulfonate (DONS) and de-epoxy-DON for porcine peripheral blood mononuclear cells and the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2, and on effects of DON and DONS on piglets
【24h】

Studies on the toxicity of deoxynivalenol (DON), sodium metabisulfite, DON-sulfonate (DONS) and de-epoxy-DON for porcine peripheral blood mononuclear cells and the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2, and on effects of DON and DONS on piglets

机译：脱氧雪腐酚（DON），焦亚硫酸钠，DON-磺酸盐（DONS）和脱环氧DON对猪外周血单个核细胞和肠道猪上皮细胞系IPEC-1和IPEC-J2的毒性研究仔猪的DON和DONS

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The in vitro effects of deoxynivalenol (DON), de-epoxy-DON, DON-sulfonate (DONS) and sodium metabisulfite (Na_2S_2O_5, SBS) on porcine peripheral blood mononuclear cells (PBMC), and on the Intestinal Porcine Epithelial Cell lines IPEC-1 and IPEC-J2 were examined by using the MTT assay.In addition, an uncontaminated and a DON contaminated triticale were included in diets either untreated (CON, FUS) or SBS treated (CON-SBS, FUS-SBS) and fed to piglets for 28 d starting from weaning. The diet concentrations of DON and DONS amounted to 0.156, 2.312, 0.084 and 0.275 mg and to <0.05, <0.05, <0.05 and 1.841 mg/kg, respectively. PBMC of the so-exposed piglets were also subjected to the MTT assay.Neither DONS and SBS nor de-epoxy-DON affected the viability of PBMC, IPEC-1 and 1PEC-J2 significantly up to concentrations of 17, 8 and 23 u.M, respectively. For DON, IC_(50) values were estimated at 1.2 +- 0.1, 1.3 + 0.5 and 3.0 +- 0.8 uM for PBMC, IPEC-1 and IPEC-J2, respectively.PBMC from piglets fed the SBS treated diets were characterized by a significantly decreased stimulation index and an increased IgA supernatant concentration with the SBS effect being significantly more pronounced after feeding the FUS-SBS diet. Further studies should clarify the possible impact of SBS on the porcine immune system.
机译：脱氧雪腐酚（DON），脱环氧DON，DON磺酸盐（DONS）和偏亚硫酸氢钠（Na_2S_2O_5，SBS）对猪外周血单核细胞（PBMC）和肠道猪上皮细胞系IPEC-的体外作用用MTT法检测1和IPEC-J2，此外，未经处理（CON，FUS）或经SBS处理（CON-SBS，FUS-SBS）的日粮中包括未污染和受DON污染的黑小麦，并喂给仔猪从断奶开始持续28天。日粮中DON和DONS的浓度分别为0.156、2.312、0.084和0.275 mg，分别为<0.05，<0.05，<0.05和1.841 mg / kg。如此暴露的仔猪的PBMC也进行了MTT分析.DONS和SBS或脱环氧DON均未显着影响PBMC，IPEC-1和1PEC-J2的活力，直至浓度为17、8和23 uM，分别。对于DON，PBMC，IPEC-1和IPEC-J2的IC_（50）值分别估计为1.2 +-0.1、1.3 + 0.5和3.0 +-0.8 uM。喂食FUS-SBS日粮后，刺激指数显着降低，IgA上清液浓度升高，SBS效应明显更为明显。进一步的研究应阐明SBS对猪免疫系统的可能影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号