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首页> 外文期刊>Food analytical methods >Quantification of Se-methylselenocysteine and its gamma-glutamyl derivative from naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) after HPLC-ESI-TOF-MS and Orbitrap MS n -based identification.
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Quantification of Se-methylselenocysteine and its gamma-glutamyl derivative from naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) after HPLC-ESI-TOF-MS and Orbitrap MS n -based identification.

机译：经过HPLC-ESI-TOF-MS和基于Orbitrap MS n的鉴定后，对天然富硒的绿豆（Phaseolus vulgaris vulgaris）中的Se-甲基硒代半胱氨酸及其γ-谷氨酰基衍生物进行定量。

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摘要

Orthogonal liquid chromatographic (ion exchange, reversed phase, and ion pairing) and mass spectrometric [electrospray ionization (ESI)-TOF-MS, ESI-Orbitrap MS, and inductively coupled plasma mass spectrometry (ICP-MS)] methods were addressed to identify and quantify selenium species from a naturally Se-enriched green bean (Phaseolus vulgaris vulgaris) sample after proteolytic digestion. While selenomethionine (10.1 mg/kg as Se) and selenate (9.5 mg/kg as Se) could be quantified in a straightforward way by anion exchange LC-ICP-MS technique, a multistep purification protocol was required to identify Se-methylselenocysteine and gamma-glutamyl-Se-methylselenocysteine in an unambiguous way prior to quantification by using either in-source fragmentation (LC-ESI-TOF-MS) or collision-induced dissociation (LC-ESI-Orbitrap MS). Finally, Se-methylselenocysteine (2.6 mg/kg as Se) and gamma-glutamyl-Se-methylselenocysteine (1.2 mg/kg as Se) could contribute to the overall selenium recovery of 72 %. This sample is the first of the Faboideae subfamily and Phaseolus ssp. to be speciated to such an extent for selenium including gamma-glutamyl-Se-methylselenocysteine, a highly potential selenium species, which makes this bean material an ideal candidate for functional food purposes. copyright Springer Science+Business Media New York 2013.
机译：解决了正交液相色谱（离子交换，反相和离子对）和质谱[电喷雾电离（ESI）-TOF-MS，ESI-Orbitrap MS和电感耦合等离子体质谱（ICP-MS）]方法的问题并通过蛋白水解消化后，从富含天然硒的绿豆（菜豆）样品中定量硒种类。尽管硒代蛋氨酸（10.1 mg / kg，硒）和硒酸盐（9.5 mg / kg，硒）可以通过阴离子交换LC-ICP-MS技术直接定量，但需要多步纯化方案来鉴定Se-甲基硒代半胱氨酸和γ -谷氨酰胺-Se-甲基硒代半胱氨酸在定量之前通过使用源内裂解（LC-ESI-TOF-MS）或碰撞诱导解离（LC-ESI-Orbitrap MS）进行明确鉴定。最后，Se-甲基硒代半胱氨酸（硒为2.6 mg / kg）和γ-谷氨酰-Se-甲基硒代半胱氨酸（硒为1.2 mg / kg）可能对总体硒回收率贡献72％。该样品是Faboideae亚科和Phaseolus ssp的第一个。对于硒的特定含量，包括γ-谷氨酰基-Se-甲基硒代半胱氨酸，硒是一种高度潜在的硒，这使得这种豆类材料成为功能食品的理想候选者。版权所有Springer Science + Business Media纽约，2013年。

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