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首页> 外文期刊>Canadian journal of microbiology >Pulsed-field gel electrophoresis typing, antibiotic resistance, and plasmid profiles of Escherichia coli strains isolated from foods
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Pulsed-field gel electrophoresis typing, antibiotic resistance, and plasmid profiles of Escherichia coli strains isolated from foods

机译：从食品中分离的大肠杆菌菌株的脉冲场凝胶电泳分型，抗生素抗性和质粒图谱

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摘要

Bacterial contamination in foods and antimicrobial resistance levels of common pathogenic strains causing foodborne disease are important in human health. Thus, typing technologies are important tools to determine primary sources of bacterial contamination. In this study, 40 Escherichia coli strains isolated from 85 food samples were evaluated in terms of genetic diversity, susceptibility to certain antibiotics, and plasmid profiles. Pulsed-field gel electrophoresis was used to identify the genetic relations of E. coli isolates. It was determined that the 40 E. coli strains revealed 32 different pulsotypes represented by 6 subtypes. Antibiotic susceptibility tests conducted by using a disc diffusion method against 15 antibiotics showed that although the isolates revealed 14 different types of resistance profiles, the strains showed the greatest resistance to ampicillin (77.5%), followed by ticarcillin- clavulanic acid (30%), tetracycline (22.5%), and cephalothin (14.5%). Plasmid isolations studies of the strains conducted by the method of alkaline lysis revealed that 18 (45%) of 40 E. coli strains contain 31 different plasmid bands ranging between 64.4 and 1 kb. The results showed that PFGE was a powerful method in tracking sources of food contamination and that the antibiotic resistance levels of food isolates were high and should be monitored.
机译：食品中的细菌污染和引起食源性疾病的常见致病菌株的抗药性水平对人类健康至关重要。因此，打字技术是确定细菌污染主要来源的重要工具。在这项研究中，从遗传多样性，对某些抗生素的敏感性和质粒谱方面评估了从85个食品样本中分离出的40株大肠杆菌。脉冲场凝胶电泳用于鉴定大肠杆菌分离株的遗传关系。已确定40个大肠杆菌菌株显示了由6个亚型代表的32种不同的脉冲型。通过圆盘扩散法对15种抗生素进行的药敏试验表明，尽管分离株显示出14种不同类型的耐药性，但菌株对氨苄青霉素的耐药性最大（77.5％），其次是替卡西林-克拉维酸（30％），四环素（22.5％）和头孢菌素（14.5％）。通过碱裂解法对菌株进行的质粒分离研究表明，40株大肠杆菌中有18个（45％）含有31个不同的质粒带，范围在64.4和1 kb之间。结果表明，PFGE是追踪食品污染源的有效方法，食品分离株的抗生素耐药性水平很高，应加以监测。

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