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首页> 外文期刊>Gynecological endocrinology: the official journal of the International Society of Gynecological Endocrinology >Nitric oxide detection by DAF (diaminofluorescein) fluorescence in human myometrial tissue.
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Nitric oxide detection by DAF (diaminofluorescein) fluorescence in human myometrial tissue.

机译：通过DAF（二氨基荧光素）荧光检测人的子宫肌层组织中的一氧化氮。

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摘要

Nitric oxide (NO) is considered to be involved in the modulation of uterine contractility. In the present pilot study, the direct detection of intracellular NO in pregnant human myometrial tissues has been investigated by using the fluorescent indicator 4,5-diaminofluorescein-2 diacetate (DAF-2DA). Pregnant myometrial tissue samples were obtained during Cesarean sections between week 34 and 40 of gestation before the onset of labor. Living explants were loaded with 10 microM DAF-2DA, stimulated with 1 mM arginine, subsequently fixed with glutaraldehyde and examined by fluorescence microscopy. The presence of NO synthases (NOS) was studied by immunohistochemistry. After application of DAF-2DA, DAF fluorescence was located primarily in blood vessels and to a minor extent in myometrial cells. By immunohistochemistry, strong endothelial NOS (eNOS) staining was found in vessel walls. In myometrial cells weak staining of eNOS and inducible NOS was observed. We conclude that the direct NO detection by DAF-2DAprovides a new and independent method to identify sites of NO production in myometrium and other heterogeneous tissues.
机译：一氧化氮（NO）被认为与子宫收缩力的调节有关。在当前的先导研究中，已经通过使用荧光指示剂4,5-二氨基荧光素-2双乙酸盐（DAF-2DA）来研究直接检测怀孕的人子宫肌组织中的细胞内NO。在分娩开始前的第34至40周的剖宫产期间，获取了妊娠子宫肌层组织样本。活的外植体装有10 microM DAF-2DA，用1 mM精氨酸刺激，随后用戊二醛固定，并通过荧光显微镜检查。通过免疫组织化学研究了NO合酶（NOS）的存在。施用DAF-2DA后，DAF荧光主要位于血管中，而在子宫肌层细胞中则较小。通过免疫组织化学，在血管壁中发现了强烈的内皮NOS（eNOS）染色。在子宫肌层细胞中观察到eNOS和诱导型NOS的弱染色。我们得出的结论是，通过DAF-2DA进行的直接NO检测提供了一种新的独立方法来鉴定子宫肌层和其他异质组织中NO的产生位置。

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