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首页> 外文期刊>Folia histochemica et cytobiologica >Correlation between NADPH-diaphorase and iNOS in bank vole Leydig cells in vitro and in testicular sections with the use of histochemistry and immunocytochemistry.
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Correlation between NADPH-diaphorase and iNOS in bank vole Leydig cells in vitro and in testicular sections with the use of histochemistry and immunocytochemistry.

机译：利用组织化学和免疫细胞化学技术，体外和睾丸切片中田鼠Leydig细胞中NADPH-心肌黄递酶与iNOS之间的相关性。

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摘要

Histochemistry for NADPH-diaphorase detects an enzymatic activity associated with nitric oxide synthase while immunohistochemistry detects the nitric oxide synthase molecule. NADPH-diaphorase and inducible isoform of nitric oxide synthase in Leydig cells in vitro and in testis sections of the bank vole were demonstrated histochemically and immunocytochemically. Histochemical studies revealed localization of NADPH-diaphorase reaction product in the cytoplasm of cultured Leydig cells as well as in the interstitial area, mainly in Leydig cells and in vascular endothelium. Distribution pattern of NADPH-diaphorase was different in Leydig cell cytoplasm of individual cells. Using immunocytochemistry, the immunoreactivity for nitric oxide synthase was observed both in cultured Leydig cells and testis sections. Moreover, a co-localization of positively immunostained cells with those histochemically detected was noticed. Addition of hCG to the cultured medium or injections in vivo resulted in a small decrease in reaction intensity in Leydig cells. Treatment with N omega-nitro-L-arginine methyl ester resulted in distinctly weaker reactivity of the enzymes studied which was correlated with a higher testosterone and estradiol levels in Leydig cells measured radioimmunologically. The results have indicated that nitric oxide synthase is able to act directly within the male gonad regulating androgen secretion by Leydig cells.
机译：NADPH-黄递酶的组织化学检测与一氧化氮合酶相关的酶活性，而免疫组织化学检测一氧化氮合酶分子。在组织化学和免疫细胞化学方面，在体外和田鼠睾丸切片中，在睾丸间质细胞中NADPH-心肌黄递酶和一氧化氮合酶的诱导型亚型都得到了证实。组织化学研究表明，NADPH-黄递酶反应产物在培养的Leydig细胞的细胞质以及间质区域定位，主要在Leydig细胞和血管内皮中。单个细胞的莱迪克细胞质中NADPH-心肌黄递酶的分布模式是不同的。使用免疫细胞化学，在培养的睾丸间质细胞和睾丸切片中均观察到一氧化氮合酶的免疫反应性。此外，注意到免疫阳性染色的细胞与组织化学检测的细胞共定位。向培养液中添加hCG或体内注射会导致Leydig细胞的反应强度略有下降。用Nω-硝基-L-精氨酸甲酯处理会明显降低所研究酶的反应性，这与放射免疫法测得的Leydig细胞中较高的睾丸激素和雌二醇水平有关。结果表明，一氧化氮合酶能够在Leydig细胞调节雄性腺的雄激素分泌中直接起作用。

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