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首页> 外文期刊>Folia histochemica et cytobiologica >A multiple marker analysis of apoptosis-associated protein expression in non-small cell lung cancer in a Chinese population
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A multiple marker analysis of apoptosis-associated protein expression in non-small cell lung cancer in a Chinese population

机译：中国人群非小细胞肺癌细胞凋亡相关蛋白表达的多标记分析

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A failure to undergo apoptosis is widely thought to be an important event in cancer formation and progression. Although there have been many studies in vitro that provide evidence for this suggestion, the roles of apoptosis-associated proteins in cancer tissues in vivo are not as yet fully understood. Moreover, multiple marker analyses of apoptosis-associated protein expression in non-small cell lung cancer (NSCLC) tissues are scarce. In the present study, we investigate the expression of a group of apoptosis-associated proteins including bcl-2, caspase-3, fas, fas ligand (fasL) and survivin, and its clinical significance in NSCLC tissues using immunohistochemistry (IHC). Bcl-2 staining in cancer tissue cells was found in cytoplasm and the positive rate was 38.2% (29/76). Caspase-3 staining was mainly seen in cytoplasm of cancer tissue cells (53.9% [41/76]) with a few cases of nuclear staining (6.6% [5/76]). Fas staining was seen in cytomembrane (15.8% [12/76]) and cytoplasm (42.1% [32/76]) of cancer tissue cells. Likewise, fasL also showed staining in cytoplasm (55.3% [42/76]) and cytomembrane (44.7% [34/76]) of cancer tissue cells. Survivin staining was seen in cytoplasm but not nuclear of cancer tissue cells and the positive rate was 48.7% (37/76). Higher cytoplasm expression of bcl-2 was associated with large tumor size (≥ 3cm) in NSCLC (p 0.05). Decreased cytoplasm expression of fas was associated with poor grade in NSCLC (p 0.05). A negative correlation was found between bcl-2 and cytoplasm caspase-3 expression in NSCLC (p 0.001). No separate expression of the apoptosis-associated proteins in NSCLC was linked to overall survival of patients (p 0.05). Multiple marker analyses revealed caspase-3 +/cytomembrane fasL - to be linked to better survival of patients with NSCLC (p 0.05). These results indicate that apoptosis- -associated proteins may impact a variety of clinicopathological features of NSCLC and may co-operatively influence the prognosis of patients with this malignant tumor.
机译：人们普遍认为，细胞凋亡失败是癌症形成和发展中的重要事件。尽管有许多体外研究为这一建议提供了证据，但凋亡相关蛋白在体内癌组织中的作用尚未得到充分了解。此外，在非小细胞肺癌（NSCLC）组织中缺乏与凋亡相关蛋白表达的多种标记分析。在本研究中，我们使用免疫组织化学（IHC）研究了一组凋亡相关蛋白（包括bcl-2，caspase-3，fas，fas配体（fasL）和survivin）的表达及其在NSCLC组织中的临床意义。在细胞质中发现癌组织细胞中Bcl-2染色，阳性率为38.2％（29/76）。 Caspase-3染色主要见于癌组织细胞的细胞质中（53.9％[41/76]），少数病例出现核染色（6.6％[5/76]）。 Fas染色可见于癌组织细胞的细胞膜（15.8％[12/76]）和细胞质（42.1％[32/76]）。同样，fasL还显示出癌组织细胞的细胞质（55.3％[42/76]）和细胞膜（44.7％[34/76]）染色。 Survivin染色可见于细胞质中，而未见癌组织细胞核，阳性率为48.7％（37/76）。 bcl-2的较高细胞质表达与NSCLC中肿瘤大（≥3cm）有关（p <0.05）。在非小细胞肺癌中，fas的胞质表达降低与不良评分有关（p <0.05）。在NSCLC中，bcl-2和细胞质caspase-3表达之间呈负相关（p <0.001）。在NSCLC中没有凋亡相关蛋白的单独表达与患者的总生存相关（p> 0.05）。多个标记分析显示，caspase-3 + /细胞膜fasL-与NSCLC患者的更好生存相关（p <0.05）。这些结果表明，凋亡相关蛋白可能影响NSCLC的多种临床病理特征，并且可以合作地影响该恶性肿瘤患者的预后。

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