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首页> 外文期刊>Folia histochemica et cytobiologica >CD3 receptor modulation in Jurkat leukemic cell line.
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CD3 receptor modulation in Jurkat leukemic cell line.

机译：Jurkat白血病细胞系中的CD3受体调节。

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CD3 antigen is a crucial molecule in T cell signal transduction. Although its expression on cell surface is constitutive, dynamic regulation of TCR-CD3 level is probably the most important mechanism allowing T cells to calibrate their response to different levels of stimuli. In our study we examined the role of two main T cell signal transduction pathways in controlling the surface level of CD3 antigen, one based on protein kinase C activity and the other dependent on calcineurin. As an experimental model we used three clones derived from Jurkat cell line, expressing different levels of CD3 antigen surface expression: CD3(low) (217.6), CD3+(217.9) or CD3(low) (217.7). The cells were stimulated with PMA or ionomycin, acting directly on PKC and calcineurin, respectively. Prior to the stimulation cells were incubated with PKC inhibitor--chelerythrine or calcineurin blocker--cyclosporine A. Changes in CD3 surface expression were measured by flow cytometry. Only PMA and chelerythrine were able to change CD3expression suggesting important involvement of PKC in the regulation of its expression. To confirm these findings, PKC activity was estimated in Jurkat clones. Our data demonstrated that Jurkat clones with different CD3 expression showed also different PKC activities, so we conclude that PKC-dependent pathway is the main way of controlling CD3 level on Jurkat clones.
机译：CD3抗原是T细胞信号转导中的关键分子。尽管其在细胞表面的表达是组成性的，但动态调节TCR-CD3水平可能是允许T细胞校准其对不同水平刺激的反应的最重要机制。在我们的研究中，我们研究了两种主要的T细胞信号转导途径在控制CD3抗原表面水平中的作用，一种基于蛋白激酶C的活性，另一种依赖于钙调神经磷酸酶。作为实验模型，我们使用了来自Jurkat细胞系的三个克隆，它们表达不同水平的CD3抗原表面表达：CD3（低）（217.6），CD3 +（217.9）或CD3（低）（217.7）。用分别直接作用于PKC和钙调神经磷酸酶的PMA或离子霉素刺激细胞。在刺激之前，将细胞与PKC抑制剂-白屈菜红碱或钙调神经磷酸酶阻滞剂-环孢菌素A孵育。通过流式细胞仪测量CD3表面表达的变化。只有PMA和白屈菜红碱能够改变CD3的表达，提示PKC参与了其表达的调控。为了证实这些发现，在Jurkat克隆中估计了PKC活性。我们的数据表明具有不同CD3表达的Jurkat克隆也表现出不同的PKC活性，因此我们得出结论，PKC依赖性途径是控制Jurkat克隆上CD3水平的主要途径。

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