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首页> 外文期刊>Forensic science international >Development of an immunoassay for the differentiation of menstrual blood from peripheral blood
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Development of an immunoassay for the differentiation of menstrual blood from peripheral blood

机译：鉴别月经血与外周血的免疫测定方法的开发

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摘要

Forensic identification of body fluids is a crucial aspect of the discipline. To date, there are no robust tests to make a distinction between menstrual blood and peripheral blood. Past techniques have fallen short of producing clear and consistent results while present mRNA techniques are still in their infancy. The aim of this study was to develop of an accurate and rapid immunoassay based identification method. Three different targets were evaluated: matrix metalloproteinase 14 (MMP14), oestrogen receptor α (ERα), and fibrinogen. Cellular or membrane bound antigens were analyzed using immunocytochemical staining while soluble antigens were analyzed by indirect ELISA. Results showed that ERα and MMP14 are present in the endometrial cells of menstrual blood but absent in peripheral blood. At a total protein concentration of 10. μg/mL or lower, fibrinogen was detected in menstrual blood but absent in peripheral. If evaluated on a larger scale, immunoassays may prove to be beneficial in discriminating menstruum and peripheral blood.
机译：法医鉴定体液是该学科的关键方面。迄今为止，还没有可靠的测试来区分月经血和外周血。过去的技术未能产生清晰一致的结果，而目前的mRNA技术仍处于起步阶段。这项研究的目的是开发一种准确，快速的基于免疫测定的识别方法。评价了三个不同的靶标：基质金属蛋白酶14（MMP14），雌激素受体α（ERα）和纤维蛋白原。细胞或膜结合抗原使用免疫细胞化学染色进行分析，而可溶性抗原则通过间接ELISA分析。结果表明，ERα和MMP14存在于月经血的子宫内膜细胞中，而在外周血中则不存在。当总蛋白浓度为10μg/ mL或更低时，在月经血中检出了纤维蛋白原，而外周血中则未检出。如果进行更大范围的评估，免疫测定可能被证明在区分月经和外周血方面是有益的。

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