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首页> 外文期刊>Food Technology and Biotechnology >Antimicrobial activity of Bacillus sp. natural isolates and their potential use in the biocontrol of phytopathogenic bacteria.
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Antimicrobial activity of Bacillus sp. natural isolates and their potential use in the biocontrol of phytopathogenic bacteria.

机译：芽孢杆菌的抗菌活性。天然分离物及其在植物致病菌生物防治中的潜在用途。

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Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.
机译：203 芽孢杆菌 sp。的筛选。对植物病原菌具有抗菌活性的天然分离物显示，经测试的127个菌株可抑制至少一种敏感菌株，这说明它们可作为生物防治剂。其中有104株分离株对米黄单胞菌（p。xanthomonas oryzae）有明显的拮抗作用。 oryzae ，其中只有一个（VPS50.2）合成细菌素。另一项筛选测试了51个分离株是否包含与iturin和surfactin类脂肽的生物合成有关的基因。结果表明，有33个分离物带有操纵子，可用于合成ururin，其中6个带有 sfp 基因，负责表面活性素的生物合成。使用乙酸乙酯萃取，超滤和反相HPLC从分离物SS12.9（确定为枯草芽孢杆菌或解淀粉芽孢杆菌）的上清液中纯化脂肽。质谱分析证实，分离物SS12.9产生了具有iX生物控制潜力的伊图林类物质。稻米 oryzae 。

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