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Botanical DNA evidence in criminal cases: Knotgrass (Polygonum aviculare L.) as a model species

机译:刑事案件中的植物DNA证据:虎杖(Polygonum aviculare L.)为模型物种

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摘要

The possibilities and strategies for using DNA characteristics to link a botanical sample to a specific source plant or location vary with its breeding system. For inbreeding species, which often form small patches of identical genotypes, knotgrass (Polygonum aviculare L.) is a suitable model species because of its (1) occurrence in a wide range of natural environments, (2) abundant presence in pieces of evidence, and (3) ease in molecular processing. The value of knotgrass for forensic casework was demonstrated using data from a homicide case. Using the DNA fingerprinting technique AFLP ? we were able to identify the knotgrass population at the crime site as the most likely origin of the botanical evidence. We expect that the development of tailored marker systems for knotgrass and other frequently occurring (model) species will considerably accelerate the use of botanical DNA evidence in criminal cases.
机译:利用DNA特性将植物样品与特定来源植物或位置联系起来的可能性和策略因其育种系统而异。对于通常形成相同基因型小斑块的近交物种,虎杖(Polygonum aviculare L.)是一种合适的模式物种,因为它具有以下优点:(1)在广泛的自然环境中发生,(2)在证据中大量存在, (3)易于分子加工。用凶杀案的数据证明了虎杖在法医案件中的价值。使用DNA指纹技术AFLP吗?我们能够确定犯罪现场的虎杖种群是最有可能的植物证据来源。我们期望针对虎杖和其他常见(模型)物种量身定制的标记系统的开发将大大加快在刑事案件中使用植物DNA证据的速度。

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