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首页> 外文期刊>Forensic science international >Technical Note: 'Mitochondrial and nuclear DNA approaches for reliable identification of Lucilia (Diptera, Calliphoridae) species of forensic interest from Southern Europe''
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Technical Note: 'Mitochondrial and nuclear DNA approaches for reliable identification of Lucilia (Diptera, Calliphoridae) species of forensic interest from Southern Europe''

机译：技术说明：“线粒体和核DNA方法可可靠地鉴定来自南欧的法医感兴趣的卢西利亚（双翅目，Calliphoridae）种”

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In forensic entomology, rapid and unambiguous identification of blowfly species is a critical prerequisite for accurately estimating the post-mortem interval (PMI). The conventional diagnosis of cadaveric entomofauna based on external characters is hampered by the morphological similarities between species, especially in immature stages. Genetic analysis has been shown to allow precise and reliable diagnosis and delimitation of insect species. Nevertheless, the taxonomy of some species remains unresolved. This study was focused on improving the effectiveness and accuracy of analysis based on the widely used cytochrome c oxidase subunit I barcode region (COI barcode, 658 bp), complemented by other mitochondrial and nuclear regions, such as cytochrome b (Cyt-b, 307 bp) and the second internal transcribed spacer (ITS2, 310-331 bp), for the identification of Southern European blowflies. We analyzed a total of 209 specimens, collected from 38 human corpses, belonging to three Calliphoridae genera and seven species: Chrysomya (Ch. albiceps), Calliphora (C. vicina and C. vomitoria), and Lucilia (L. sericata, L. ampullacea, L. caesar and L. illustris). These species are the most common PMI indicators in Portugal. The results revealed that unambiguous separation of species of the Lucilia genus requires different loci from the barcode region. Furthermore, we conclude that the ITS2 (310-331 bp) molecular marker is a promising diagnostic tool because its inter-specific discriminatory power enables unequivocal and consistent distinctions to be made, even between closely related species (L. caesar-L. illustris). This work also contributes new genetic data that may be of interest in performing species diagnosis for Southern European blowflies. Notably, to the best of our knowledge, we provide the first records of the Cyt-b (307 bp) locus for L. illustris and the ITS2 (310-331 bp) region for Iberian Peninsula Lucilia species. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
机译：在法医昆虫学中，快速明确地确定蝇类是准确估计尸检间隔（PMI）的关键前提。基于外部特征的尸体昆虫动物的常规诊断受到物种之间形态相似性的阻碍，特别是在未成熟阶段。遗传分析已被证明可以对昆虫种类进行精确可靠的诊断和定界。尽管如此，某些物种的分类学仍未解决。这项研究的重点是基于广泛使用的细胞色素c氧化酶亚基I条码区域（COI条码，658 bp），辅以其他线粒体和核区域，如细胞色素b（Cyt-b，307）来提高分析的效率和准确性。 bp）和第二个内部转录间隔区（ITS2，310-331 bp），用于鉴定南欧苍蝇。我们分析了总共209个标本，这些标本从38个人体尸体中收集，分别属于3个Calliphoridae属和7个物种：Chrysomya（Ch。albiceps），Calliphora（C. vicina和C. vomitoria）和Lucilia（L. sericata，L.壶腹，凯撒乳杆菌和illusillus）。这些物种是葡萄牙最常见的PMI指标。结果表明，明确区分Lucilia属的物种需要与条形码区域不同的基因座。此外，我们得出结论，ITS2（310-331 bp）分子标记物是一种很有前途的诊断工具，因为它的种间区分能力使即使在密切相关的物种之间也能进行明确而一致的区分（L. caesar-L。illustris） 。这项工作还提供了新的遗传数据，可能对执行南欧苍蝇的物种诊断感兴趣。值得注意的是，据我们所知，我们提供了illus L. illust的Cyt-b（307 bp）位点和伊比利亚半岛Lucilia物种的ITS2（310-331 bp）区域的第一个记录。 （C）2015 Elsevier Ireland Ltd.保留所有权利。

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