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首页> 外文期刊>Folia neuropathologica >Protective effects of peel and seed extracts of Citrus aurantium on glutamate-induced cytotoxicity in PC12 cell line
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Protective effects of peel and seed extracts of Citrus aurantium on glutamate-induced cytotoxicity in PC12 cell line

机译：ur皮和种子提取物对谷氨酸诱导的PC12细胞毒性的保护作用

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摘要

Oxidative stress and apoptosis contribute to neuronal degeneration in many neurodegenerative diseases such as Alzheimer's disease. Glutamate is a major excitatory neurotransmitter in the central nervous system (CNS) and is considered responsible for the pathogenesis of many neurological disorders. Reactive oxygen species (ROS) production is thought to be involved in glutamate-induced apoptosis process. In this study, the neuroprotective effects of Citrus auraptium in the glutamate-induced rat's adrenal pheochromocytoma cell line (PC12 cells) were investigated. The cell viability and apoptotic cell death were measured using MTT and propidium iodine (PI)-staining methods, respectively. In addition, intracellular ROS and malondialdehyde (MDA) levels were determined by fluorometric methods. The results showed that glutamate cytotoxicity in PC12 cells was accompanied by an increment of MDA content, ROS generation, and apoptotic induction. However, pretreatment with peel and seed extracts of C. aurantium significantly reduced MDA content, ROS generation, and apoptotic cells. All these findings indicated that C. aurantium protected PC12 cells against glutamate-induced apoptosis by inhibiting ROS production. Therefore, the present study supports that C. aurantium extracts possess neuroprotective effects against glutamate-induced toxicity in PC12 cell line. The protective effect of C. aurantium might be attributed to its antioxidant properties.
机译：氧化应激和细胞凋亡导致许多神经退行性疾病（例如阿尔茨海默氏病）中的神经元变性。谷氨酸是中枢神经系统（CNS）中主要的兴奋性神经递质，被认为与许多神经系统疾病的发病机理有关。活性氧（ROS）的产生被认为与谷氨酸诱导的细胞凋亡过程有关。在这项研究中，研究了柑桔橙对谷氨酸诱导的大鼠肾上腺嗜铬细胞瘤细胞系（PC12细胞）的神经保护作用。使用MTT和碘化丙锭（PI）染色法分别测量细胞活力和凋亡细胞死亡。另外，通过荧光法确定细胞内ROS和丙二醛（MDA）水平。结果显示，PC12细胞中的谷氨酸细胞毒性伴随着MDA含量，ROS生成和凋亡诱导的增加。但是，用桔梗的果皮和种子提取物进行预处理可显着降低MDA含量，ROS生成和凋亡细胞。所有这些发现表明，金黄色葡萄球菌通过抑制ROS产生来保护PC12细胞免受谷氨酸诱导的凋亡。因此，本研究支持金黄色葡萄球菌提取物对谷氨酸诱导的PC12细胞系毒性具有神经保护作用。金桔梗的保护作用可能归因于其抗氧化性能。

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