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首页> 外文期刊>Forest Pathology >Molecular phylogeny and pathotyping of Fusarium solani: a causal agent of Dalbergia sissoo decline
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Molecular phylogeny and pathotyping of Fusarium solani: a causal agent of Dalbergia sissoo decline

机译：茄镰刀菌的分子系统发育和致病类型：黄檀黄萎病的致病因子

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Sissoo (Dalbergia sissoo), commonly known as shisham, is amongst the finest woods of South Asia, but wilt' disease has caused a rapid decline in this species. The cause of the disease remains uncertain. The aim of this study is to identify the causal agent of the disease and characterize isolates made from diseased trees, based on genomic data and variations in virulence. Samples of infected roots, stems and the ooze exuded from infected trees were obtained from plants showing symptoms in different geographical regions of India for the isolation of microorganisms. Isolates were used to inoculate healthy plants. Based on the morphological characteristics, genus- and species-specific PCR, and in silico analysis of 5.8S rDNA-ITS regions, of the 38 fungal isolates, 24 and 14 were identified as Fusarium solani and Fusarium sp., respectively. In a pathotyping study, eighteen F.solani isolates, isolated from roots and stem parts of symptomatic plants, induced typical wilt symptoms when inoculated through soil and roots on D.sissoo seedlings of 1-15months in age. The population of F.solani was the highest in infected roots and the lowest in parts of stems, gradually decreasing with height, and was isolated constantly up to approximately 40% height of the seedling. F.solani isolates used in inoculations were successfully re-isolated from the rhizosphere, infected roots and wilted stems, as confirmed using isolate-specific DNA fingerprints. Molecular phylogenies based on rDNA-ITS sequences showed that the 38 isolates fell into 2 groups. Group I comprised of F.solani isolates from D.sissoo and F.solani sequences in the NCBI GenBank database, whereas group II included Fusarium isolates other than F.solani. These results are helpful in developing integrated control measures for this highly variable pathogen and to establish a base for future population studies.
机译：Sissoo（Dalbergia sissoo），通常被称为shisham，是南亚最好的森林之一，但是枯萎病导致该物种迅速减少。疾病的原因仍然不确定。这项研究的目的是基于基因组数据和毒力变化，确定该病的病原体并鉴定出患病树木的分离株。从受感染树木中渗出的被感染根，茎和渗出物的样品是从印度不同地理区域表现出症状的植物中分离出来的，这些样品表现出了微生物的分离作用。分离株用于接种健康植物。根据形态特征，属和种特异性PCR以及5.8S rDNA-ITS区域的计算机分析，在38种真菌中，分别鉴定了24种和14种分别为茄枯萎菌和镰刀菌。在一项病理分型研究中，从有症状植物的根和茎部分分离出的十八种F.solani菌株，当通过土壤和根系接种1-15个月大的D.sissoo幼苗时，会诱发典型的枯萎症状。 F.solani的种群在受感染的根中最高，在茎的一部分中最低，随高度的增加而逐渐减少，并不断地被隔离直至幼苗高度的大约40％。如使用分离物特异的DNA指纹图所示，已成功地从根际，感染的根和枯萎的茎中成功分离出了用于接种的F.solani分离物。基于rDNA-ITS序列的分子系统学研究表明38个分离株分为两组。第一组包括NCBI GenBank数据库中来自D.sissoo和F.solani序列的F.solani分离株，而第二组包括除F.solani之外的镰刀菌分离株。这些结果有助于为这种高度可变的病原体制定综合控制措施，并为将来的人群研究奠定基础。

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